Bacteriology Exam 2: Diagnostic
Methods correctly answered to pass
Bacterial Identification happens by what two methods? - correct answer ✔✔1) Phenotypic methods
2) Genotypic Methods
___________ method is based on specific characteristics displayed by the organism - correct answer
✔✔Phenotypic Methods
_______ Methods is based on genetic information contained in the DNA - correct answer ✔✔Genotypic
Methods
What is included in phenotypic characteristics? - correct answer ✔✔1) Growth characteristics
2) Cell morphology
- Size, Shape and arrangement
- Staining characteristics
3) Biochemical reactions
Phenotypic Methods: Growth Characteristics, what type of broth do we look at? - correct answer
✔✔Broth & Solid Medium
Broth: is a liquid medium (Subsp. necrophorum)
Solid Medium: (Subsp. funduliforme)
, On the solid medium, colony morphology occurs on the ________ - correct answer ✔✔Blood agar
On the solid medium, growth occurs on _______ & _______ media - correct answer ✔✔Selective &
Differential
The solid medium may provide clues to what? - correct answer ✔✔Clues to the identity of the organism
What are the clinical signs of infections of Urinary Tracts in Dogs or Cats? - correct answer ✔✔- Pain,
pus, smell, blood, etc
Urinary Tract Infections samples are plated on a ______ agar. - correct answer ✔✔Maconkey agar
MacConkey Agar is a selective medium for ______________________ - correct answer ✔✔Gram
negative enteric bacteria, like E. coli
Microscopic Morphology includes _________ & _______ - correct answer ✔✔Size & Shape
- Unstained (wet mount)
- Stained
Microscopic Morphology may be sufficient to make a ________________________ - correct answer
✔✔Presumptive diagnosis
Bacteria are colorless, so we need to _________________ - correct answer ✔✔Stain them with dyes
Bacteria dyes are either _______ or _______ - correct answer ✔✔Acidic Or Basic
Bacteria are _________ charged, hence basic dyes are more often used - correct answer ✔✔Negatively
Name 4 basic types of dyes - correct answer ✔✔1) Crystal violet
2) Methylene Blue
Methods correctly answered to pass
Bacterial Identification happens by what two methods? - correct answer ✔✔1) Phenotypic methods
2) Genotypic Methods
___________ method is based on specific characteristics displayed by the organism - correct answer
✔✔Phenotypic Methods
_______ Methods is based on genetic information contained in the DNA - correct answer ✔✔Genotypic
Methods
What is included in phenotypic characteristics? - correct answer ✔✔1) Growth characteristics
2) Cell morphology
- Size, Shape and arrangement
- Staining characteristics
3) Biochemical reactions
Phenotypic Methods: Growth Characteristics, what type of broth do we look at? - correct answer
✔✔Broth & Solid Medium
Broth: is a liquid medium (Subsp. necrophorum)
Solid Medium: (Subsp. funduliforme)
, On the solid medium, colony morphology occurs on the ________ - correct answer ✔✔Blood agar
On the solid medium, growth occurs on _______ & _______ media - correct answer ✔✔Selective &
Differential
The solid medium may provide clues to what? - correct answer ✔✔Clues to the identity of the organism
What are the clinical signs of infections of Urinary Tracts in Dogs or Cats? - correct answer ✔✔- Pain,
pus, smell, blood, etc
Urinary Tract Infections samples are plated on a ______ agar. - correct answer ✔✔Maconkey agar
MacConkey Agar is a selective medium for ______________________ - correct answer ✔✔Gram
negative enteric bacteria, like E. coli
Microscopic Morphology includes _________ & _______ - correct answer ✔✔Size & Shape
- Unstained (wet mount)
- Stained
Microscopic Morphology may be sufficient to make a ________________________ - correct answer
✔✔Presumptive diagnosis
Bacteria are colorless, so we need to _________________ - correct answer ✔✔Stain them with dyes
Bacteria dyes are either _______ or _______ - correct answer ✔✔Acidic Or Basic
Bacteria are _________ charged, hence basic dyes are more often used - correct answer ✔✔Negatively
Name 4 basic types of dyes - correct answer ✔✔1) Crystal violet
2) Methylene Blue
