Bacteriology Lab Exam questions with
100% correct answers
aseptic technique - correct answer ✔✔procedures that prevent microbes from getting where they do
not belong (contamination). This includes microbes from the environment contaminating your lab area
as well as any microbes under study contaminating you or your work area
Bibulous paper - correct answer ✔✔specially prepared blotting paper that contain a minimum of loose
paper fibers
eukaryotic - correct answer ✔✔cells w/ a true nucleus and paired chomosomes
parfocal - correct answer ✔✔feature of most microscopes that sets the focal point of all objective lenses
at the same location in space
prokaryotic - correct answer ✔✔cells w/ no true nucleus (membrane covered, paired chromosomes), w/
only one circular chromosome
resolution - correct answer ✔✔the ability to see a small object clearly under the microscope (technically,
minimal distance at which two adjacent small objects can be distinguished as separate)
rheostat - correct answer ✔✔device that controls the amount of electrical current and thus the amount
of light emanating from a bulb
agar - correct answer ✔✔solidifying agent used for growth media derived from seaweed
agar deep - correct answer ✔✔growth medium in a test tube allowed to solidify as the tube sits in a test-
tube rack
agar plate - correct answer ✔✔solidified growth medium in a lid-covered, flat dish
, agar slant - correct answer ✔✔solidified growth medium in a test tube, allowed to solidify at an angle,
thus presenting a large surface area to allow for growth
autoclave - correct answer ✔✔device that uses pressurized (15 lbs/in^2) steam at 121 degrees Celsius to
kill all known infectious agents in 15 minutes
broth - correct answer ✔✔general term for liquid growth medium
colony - correct answer ✔✔a visible clump of bacteria growing on an agar plate, separated from other
areas of growth
contamination - correct answer ✔✔presence or possible presence of microbes in an area where they do
not belong
incubation - correct answer ✔✔(in the laboratory) allowing a microbe to grow at a constant (usually
optimal) temperature
inoculating loop - correct answer ✔✔device used to aseptically transfer and streak microbes in the
laboratory
inoculation - correct answer ✔✔(in the laboratory) the process of introducing microbes into a culture
medium
inverted - correct answer ✔✔the position of a petri dish or agar plate whereby the lid side is facing
downward
petri dish - correct answer ✔✔a flat dish, w/ a lid. the base is filled w/ solidified growth medium used to
isolate and grow bacteria
pour plate - correct answer ✔✔method of separating, isolating, and counting bacteria by placing a small
sample of the microbe in a melted agar and pouring into a petri dish
100% correct answers
aseptic technique - correct answer ✔✔procedures that prevent microbes from getting where they do
not belong (contamination). This includes microbes from the environment contaminating your lab area
as well as any microbes under study contaminating you or your work area
Bibulous paper - correct answer ✔✔specially prepared blotting paper that contain a minimum of loose
paper fibers
eukaryotic - correct answer ✔✔cells w/ a true nucleus and paired chomosomes
parfocal - correct answer ✔✔feature of most microscopes that sets the focal point of all objective lenses
at the same location in space
prokaryotic - correct answer ✔✔cells w/ no true nucleus (membrane covered, paired chromosomes), w/
only one circular chromosome
resolution - correct answer ✔✔the ability to see a small object clearly under the microscope (technically,
minimal distance at which two adjacent small objects can be distinguished as separate)
rheostat - correct answer ✔✔device that controls the amount of electrical current and thus the amount
of light emanating from a bulb
agar - correct answer ✔✔solidifying agent used for growth media derived from seaweed
agar deep - correct answer ✔✔growth medium in a test tube allowed to solidify as the tube sits in a test-
tube rack
agar plate - correct answer ✔✔solidified growth medium in a lid-covered, flat dish
, agar slant - correct answer ✔✔solidified growth medium in a test tube, allowed to solidify at an angle,
thus presenting a large surface area to allow for growth
autoclave - correct answer ✔✔device that uses pressurized (15 lbs/in^2) steam at 121 degrees Celsius to
kill all known infectious agents in 15 minutes
broth - correct answer ✔✔general term for liquid growth medium
colony - correct answer ✔✔a visible clump of bacteria growing on an agar plate, separated from other
areas of growth
contamination - correct answer ✔✔presence or possible presence of microbes in an area where they do
not belong
incubation - correct answer ✔✔(in the laboratory) allowing a microbe to grow at a constant (usually
optimal) temperature
inoculating loop - correct answer ✔✔device used to aseptically transfer and streak microbes in the
laboratory
inoculation - correct answer ✔✔(in the laboratory) the process of introducing microbes into a culture
medium
inverted - correct answer ✔✔the position of a petri dish or agar plate whereby the lid side is facing
downward
petri dish - correct answer ✔✔a flat dish, w/ a lid. the base is filled w/ solidified growth medium used to
isolate and grow bacteria
pour plate - correct answer ✔✔method of separating, isolating, and counting bacteria by placing a small
sample of the microbe in a melted agar and pouring into a petri dish
