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BOC Urinalysis / Body Fluids
Questions and Answers (100% Pass)
1) After receiving a 24 hr. urine for quantitative total protein analysis the
technician must first:
a. subculture the urine for bacteria
b. add the appropriate preservative
c. screen for albumin using a dipstick
d. measure the total volume
✓ d (Measure the total volume of the sample before removing an aliquot.
To calculate the total protein, measure the protein of an aliquot to
learn the mg/dL. Then multiply that answer by the number of dL in the
24-hour collection.)
2) False results in the urobilinogen testing may occur if the urine specimen is:
a. exposed to light
b. adjusted to a neutral pH
c. cooled to room temp
d. collected in a nonsterile container
✓ a (Urobilinogen is degraded by light.)
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3) A clean catch urine is submitted to laboratory for routine urinalysis and
culture. The routine urinalysis is done first, and 3 hrs. later, the specimen is sent
to the microbiology department for culture. The specimen should:
a. be centrifuged, and the supernatant culture
b. be rejected due to the time delay
c. not be cultured if no bacteria are seen
d. be processed for culture only if the nitrate is positive
✓ b (It is common practice to share samples between the microbiology
department and urinalysis. Ideally, the culture is set up first to prevent
contamination. If that is not feasible time wise, the sample should be
aliquotted using aseptic technique, and refrigerated until it can be
cultured.)
4) Which of the following urine results is most apt to be changed by
prolonged exposure to light?
a. pH
b. protein
c. ketones
d. bilirubin
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✓ d (Bilirubin is degraded by light. The other analytes will not be
affected.)
5) Urine samples should be examined within 1 hr. of voiding because:
a. RBC's, leukocytes and casts agglutinate on standing for several hrs. at
room temp
b. urobilinogen increases and bilirubin decreases after prolonged exposure
to light
c. bacterial contamination will cause alkalinization of the urine
d. ketones will increase due to bacterial and cellular metabolism
✓ c (Evaluate each statement. a is incorrect because these cells don't
agglutinate. b is partially correct, but urobilinogen decreases in light. c
is true, bacterial overgrowth does lead to an alkaline urine. d is false,
ketones are produced by fat metabolism in the patient.)
6) If a urine specimen was stored uncapped at 5 degrees C without
preservation and retested at 2pm which of the following test results would be
changed due to these storage conditions?
A. glucose
B. ketones
C. protein
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D. nitrite
✓ Ketones
7) A urine specimen comes to the laboratory 7 hrs. after it is obtained. It is
acceptable for culture only if the specimen has been stored:
a. at room temp
b. at 4 - 7 C
c. frozen
d. with a preservative additive
✓ b (Storage must inhibit bacterial growth but not kill the bacteria.
Freezing and additives are not acceptable. The most commonly used
method of preservation is refrigeration.)
8) Which of the following would be affected by allowing a urine specimen to
remain at room temp for 3 hrs. before analysis:
a. occult blood
b. specific gravity
c. pH
d. protein
Master01 | October, 2024/2025 | Latest update
BOC Urinalysis / Body Fluids
Questions and Answers (100% Pass)
1) After receiving a 24 hr. urine for quantitative total protein analysis the
technician must first:
a. subculture the urine for bacteria
b. add the appropriate preservative
c. screen for albumin using a dipstick
d. measure the total volume
✓ d (Measure the total volume of the sample before removing an aliquot.
To calculate the total protein, measure the protein of an aliquot to
learn the mg/dL. Then multiply that answer by the number of dL in the
24-hour collection.)
2) False results in the urobilinogen testing may occur if the urine specimen is:
a. exposed to light
b. adjusted to a neutral pH
c. cooled to room temp
d. collected in a nonsterile container
✓ a (Urobilinogen is degraded by light.)
Master01 | October, 2024/2025 | Latest update
, 1|Page | © copyright 2024/2025 | Grade A+
3) A clean catch urine is submitted to laboratory for routine urinalysis and
culture. The routine urinalysis is done first, and 3 hrs. later, the specimen is sent
to the microbiology department for culture. The specimen should:
a. be centrifuged, and the supernatant culture
b. be rejected due to the time delay
c. not be cultured if no bacteria are seen
d. be processed for culture only if the nitrate is positive
✓ b (It is common practice to share samples between the microbiology
department and urinalysis. Ideally, the culture is set up first to prevent
contamination. If that is not feasible time wise, the sample should be
aliquotted using aseptic technique, and refrigerated until it can be
cultured.)
4) Which of the following urine results is most apt to be changed by
prolonged exposure to light?
a. pH
b. protein
c. ketones
d. bilirubin
Master01 | October, 2024/2025 | Latest update
, 1|Page | © copyright 2024/2025 | Grade A+
✓ d (Bilirubin is degraded by light. The other analytes will not be
affected.)
5) Urine samples should be examined within 1 hr. of voiding because:
a. RBC's, leukocytes and casts agglutinate on standing for several hrs. at
room temp
b. urobilinogen increases and bilirubin decreases after prolonged exposure
to light
c. bacterial contamination will cause alkalinization of the urine
d. ketones will increase due to bacterial and cellular metabolism
✓ c (Evaluate each statement. a is incorrect because these cells don't
agglutinate. b is partially correct, but urobilinogen decreases in light. c
is true, bacterial overgrowth does lead to an alkaline urine. d is false,
ketones are produced by fat metabolism in the patient.)
6) If a urine specimen was stored uncapped at 5 degrees C without
preservation and retested at 2pm which of the following test results would be
changed due to these storage conditions?
A. glucose
B. ketones
C. protein
Master01 | October, 2024/2025 | Latest update
, 1|Page | © copyright 2024/2025 | Grade A+
D. nitrite
✓ Ketones
7) A urine specimen comes to the laboratory 7 hrs. after it is obtained. It is
acceptable for culture only if the specimen has been stored:
a. at room temp
b. at 4 - 7 C
c. frozen
d. with a preservative additive
✓ b (Storage must inhibit bacterial growth but not kill the bacteria.
Freezing and additives are not acceptable. The most commonly used
method of preservation is refrigeration.)
8) Which of the following would be affected by allowing a urine specimen to
remain at room temp for 3 hrs. before analysis:
a. occult blood
b. specific gravity
c. pH
d. protein
Master01 | October, 2024/2025 | Latest update
