MB(ASCP) EXAM () PRACTICE

MB(ASCP) EXAM (2024-2025) PRACTICE


methods of gel staining: - <<<Answers>>>1. EtBr
2. silver staining
3. SYBR green
4. Methylene Blue


SYBR Green I and II - <<<Answers>>>I= ds DNA
II= ss and RNA
3 steps of translation - <<<Answers>>>1. initiation
2. elongation
3. termination


mode of inheritance for Huntington's disease -
<<<Answers>>>autosomal dominant


Huntington's disease - <<<Answers>>>chr 4p16
IT-15 Gene (Huntington protein)
CAG repeats


<29 = normal
29-35 = borderline
36-121 = disease

, MB(ASCP) EXAM (2024-2025) PRACTICE

mode of inheritance for cystic fibrosis -
<<<Answers>>>autosomal recessive


gene impacted in CF - <<<Answers>>>CFTR gene


Fragile X - <<<Answers>>>most common form of inherited
retardation
-FMR-1 gene
-Xq27.3
-CGG repeat


5-44 = normal
59-200 = carrier
200+ = disease
nitrocellulose membranes - <<<Answers>>>fragile, brittle
NOT for re-probing
intense color


Nylon membrane - <<<Answers>>>strong, durable
can strip and re-probe
can be charged
NOT for Western Blotting

, MB(ASCP) EXAM (2024-2025) PRACTICE

Southern Blot - <<<Answers>>>DNA


Western Blot - <<<Answers>>>protein


Northern Blot - <<<Answers>>>RNA


steps of pre-transfer for membranes - <<<Answers>>>1. de-
purinate DNA to reduce the size of fragments to transfer
2. denature DNA
3. neutralize to equlibrate gel and membrane


capillary transfer to membrane - <<<Answers>>>transfer
buffer drawn up through a reservoir and passes through gel
into a stack of paper towels weighted; DNA moves from gel
with movement of buffer


does NOT work forpolyacrylamide gels


dot blots - <<<Answers>>>bind DNA or RNA to membrane
without first separating the components by electrophoresis


less discrimination in hybridization