BOC Immunology Exam-Questions with Correct Answers/ Verified
1) Antinuclear antibody tests are performed to help diagnose?
a) acute leukemia
b) lupus erythematosus
c) hemolytic anemia
d) Crohn disease - ✔✔b (lupus erythematosus)
2. In the anti-double-stranded DNA procedure, the antigen most commonly utilized is:
a. rat stomach tissue
b. mouse kidney tissue
c. Crithida lucilia
d. Toxoplasma gondii - ✔✔c (Crithida lucilia)
3) Which of the ANA patterns shown above would be associated with high titers of
antibodies to the Sm antigen?
a) diagram A
b) diagram B
c) diagram C
d) diagram D - ✔✔c (diagram C)
4) Sera to be tested for IFA-ANA 6 days after drawing is best stored at?
a) room temperature
b) 5°C +/- 2°
c) -70°C in a constant temperature freezer
d) -20°C in a frost-free self-defrosting freezer - ✔✔b (5°C +/- 2°)
5) Antibodies directed at native DNA are most frequently associated with which pattern of
fluorescence in the IFA-ANA test?
,a) rim
b) diffuse
c) speckled
d) centromere - ✔✔a (rim)
6) The technologist observes apparent homogenous staining of the nucleus of interphase
cells while performing an IFA-ANA, as well as staining of the chromosomes in mitotic cells.
This result is:
a) indicative of 2 antibodies, which should be separately reported after titration
b) expected for anti-DNA antibodies
c) inconsistent; the test should be reported with new reagent
d) expected for anti-centromere antibodies - ✔✔b (expected for anti-DNA antibodies)
7) The result of an anti-nuclear antibody test was a titer of 1:320 with a peripheral pattern.
Which of the following sets of results best correlate with these results?
a) anti-dsDNA titer 1:80, and a high titer of antibodies to Sm
b) antimitochondrial antibody titer 1:160, and antibodies to RNP
c) anti-Scl-70, and antibodies to single-stranded DNA
d) high titers of anti-SS-A and anti-SS-B - ✔✔a (anti-dsDNA titer 1:80, and a high titer of
antibodies to Sm)
8) Systemic lupus erythematosus patients often have which of the following test results?
a) high titers of DNA antibody
b) decreased serum immunoglobulin levels
c) high titers of anti-smooth muscle antibodies
d) high titers of antimitochondrial antibody - ✔✔a (high titers of DNA antibody)
9. Systemic lupus erythematosus patients with active disease often have which of the
following test results?
a. high titers of antimicrosomal antibodies
,b. high titers of anti-smooth muscle antibodies
c. marked decrease in serum CH50
d. decreased serum immunoglobulin levels - ✔✔c (marked decrease in serum CH50)
10. Which of the following is decreased in serum during the active stages of systemic lupus
erythematosus?
a. anti-nuclear antibody
b. immune complexes
c. complement (C3)
d. anti-DNA - ✔✔c (complement (C3))
11. A positive ANA with the pattern of anticentromere antibodies is most frequently seen in
patients with:
a. rheumatoid arthritis
b. systemic lupus erythematosus
c. CREST syndrome
d. Sjӧgren syndrome - ✔✔c (CREST syndrome)
12. In the indirect fluorescent anti-nuclear antibody test, a homogenous pattern indicates
the presence of antibody to:
a. RNP
b. Sm
c. RNA
d. DNA - ✔✔DNA
13. In the indirect fluorescent anti-nuclear antibody test, a speckled pattern may indicate
the presence of antibody to:
, a. histone
b. Sm
c. RNA
d. DNA - ✔✔b (Sm)
14) A patient has the following test results:
The above results could be seen in patients with:
a) rheumatic fever
b) rheumatoid arthritis
c) lupus erythematosus
d) glomerulonephritis - ✔✔c (lupus erythematosus)
15. Autoantibodies in the absence of Sm are found in patients with:
a. mixed connective tissue disease
b. systemic lupus erythematosus
c. Crohn disease
d. multiple myeloma - ✔✔a (mixed connective tissue disease)
16) Which of the following is the most sensitive and appropriate method for the detection
of rheumatoid factor?
a) nephelometry
b) immunofixation electrophoresis
c) immunofluorescence
d) manual latex agglutination - ✔✔a (nephelometry)
17) Rheumatoid factor reacts with:
1) Antinuclear antibody tests are performed to help diagnose?
a) acute leukemia
b) lupus erythematosus
c) hemolytic anemia
d) Crohn disease - ✔✔b (lupus erythematosus)
2. In the anti-double-stranded DNA procedure, the antigen most commonly utilized is:
a. rat stomach tissue
b. mouse kidney tissue
c. Crithida lucilia
d. Toxoplasma gondii - ✔✔c (Crithida lucilia)
3) Which of the ANA patterns shown above would be associated with high titers of
antibodies to the Sm antigen?
a) diagram A
b) diagram B
c) diagram C
d) diagram D - ✔✔c (diagram C)
4) Sera to be tested for IFA-ANA 6 days after drawing is best stored at?
a) room temperature
b) 5°C +/- 2°
c) -70°C in a constant temperature freezer
d) -20°C in a frost-free self-defrosting freezer - ✔✔b (5°C +/- 2°)
5) Antibodies directed at native DNA are most frequently associated with which pattern of
fluorescence in the IFA-ANA test?
,a) rim
b) diffuse
c) speckled
d) centromere - ✔✔a (rim)
6) The technologist observes apparent homogenous staining of the nucleus of interphase
cells while performing an IFA-ANA, as well as staining of the chromosomes in mitotic cells.
This result is:
a) indicative of 2 antibodies, which should be separately reported after titration
b) expected for anti-DNA antibodies
c) inconsistent; the test should be reported with new reagent
d) expected for anti-centromere antibodies - ✔✔b (expected for anti-DNA antibodies)
7) The result of an anti-nuclear antibody test was a titer of 1:320 with a peripheral pattern.
Which of the following sets of results best correlate with these results?
a) anti-dsDNA titer 1:80, and a high titer of antibodies to Sm
b) antimitochondrial antibody titer 1:160, and antibodies to RNP
c) anti-Scl-70, and antibodies to single-stranded DNA
d) high titers of anti-SS-A and anti-SS-B - ✔✔a (anti-dsDNA titer 1:80, and a high titer of
antibodies to Sm)
8) Systemic lupus erythematosus patients often have which of the following test results?
a) high titers of DNA antibody
b) decreased serum immunoglobulin levels
c) high titers of anti-smooth muscle antibodies
d) high titers of antimitochondrial antibody - ✔✔a (high titers of DNA antibody)
9. Systemic lupus erythematosus patients with active disease often have which of the
following test results?
a. high titers of antimicrosomal antibodies
,b. high titers of anti-smooth muscle antibodies
c. marked decrease in serum CH50
d. decreased serum immunoglobulin levels - ✔✔c (marked decrease in serum CH50)
10. Which of the following is decreased in serum during the active stages of systemic lupus
erythematosus?
a. anti-nuclear antibody
b. immune complexes
c. complement (C3)
d. anti-DNA - ✔✔c (complement (C3))
11. A positive ANA with the pattern of anticentromere antibodies is most frequently seen in
patients with:
a. rheumatoid arthritis
b. systemic lupus erythematosus
c. CREST syndrome
d. Sjӧgren syndrome - ✔✔c (CREST syndrome)
12. In the indirect fluorescent anti-nuclear antibody test, a homogenous pattern indicates
the presence of antibody to:
a. RNP
b. Sm
c. RNA
d. DNA - ✔✔DNA
13. In the indirect fluorescent anti-nuclear antibody test, a speckled pattern may indicate
the presence of antibody to:
, a. histone
b. Sm
c. RNA
d. DNA - ✔✔b (Sm)
14) A patient has the following test results:
The above results could be seen in patients with:
a) rheumatic fever
b) rheumatoid arthritis
c) lupus erythematosus
d) glomerulonephritis - ✔✔c (lupus erythematosus)
15. Autoantibodies in the absence of Sm are found in patients with:
a. mixed connective tissue disease
b. systemic lupus erythematosus
c. Crohn disease
d. multiple myeloma - ✔✔a (mixed connective tissue disease)
16) Which of the following is the most sensitive and appropriate method for the detection
of rheumatoid factor?
a) nephelometry
b) immunofixation electrophoresis
c) immunofluorescence
d) manual latex agglutination - ✔✔a (nephelometry)
17) Rheumatoid factor reacts with:
