Carbohydrate/Glucose Metabolism
(Glycolysis)
, Glycolysis: An Overview
• Glycolysis, the major pathway for glucose
oxidation, occurs in the cytosol of all cells.
• It is unique, in that it can function either
aerobically or anaerobically, depending on the
availability of oxygen and intact mitochondria.
• It allows tissues to survive in presence or absence
of oxygen, e.g., skeletal muscle.
• Red blood cells (RBCs), which lack mitochondria,
are completely reliant on glucose as their
metabolic fuel, and metabolizes it by anaerobic
glycolysis
,Glycolysis
, Pyruvate Kinase Deficiency - Hemolytic Anemia
• Pyruvate kinase (PK) is an enzyme that
catalyzes the conversion of
phosphoenolpyruvate and ADP to pyruvate and
ATP in glycolysis and plays a role in regulating
cell metabolism
PK Mutation may lead to:
1. Altered Enz. Kinetics.
2. Altered response to activator.
3. Decreased the amount of the Enz. or its stability
• Vmax is the reaction rate when the enzyme is
fully saturated by substrate, indicating that all
the binding sites are being constantly
reoccupied
• Km substrate concentration at which the
reaction rate is 50% of the Vmax. A measure of
the affinity an enzyme
(Glycolysis)
, Glycolysis: An Overview
• Glycolysis, the major pathway for glucose
oxidation, occurs in the cytosol of all cells.
• It is unique, in that it can function either
aerobically or anaerobically, depending on the
availability of oxygen and intact mitochondria.
• It allows tissues to survive in presence or absence
of oxygen, e.g., skeletal muscle.
• Red blood cells (RBCs), which lack mitochondria,
are completely reliant on glucose as their
metabolic fuel, and metabolizes it by anaerobic
glycolysis
,Glycolysis
, Pyruvate Kinase Deficiency - Hemolytic Anemia
• Pyruvate kinase (PK) is an enzyme that
catalyzes the conversion of
phosphoenolpyruvate and ADP to pyruvate and
ATP in glycolysis and plays a role in regulating
cell metabolism
PK Mutation may lead to:
1. Altered Enz. Kinetics.
2. Altered response to activator.
3. Decreased the amount of the Enz. or its stability
• Vmax is the reaction rate when the enzyme is
fully saturated by substrate, indicating that all
the binding sites are being constantly
reoccupied
• Km substrate concentration at which the
reaction rate is 50% of the Vmax. A measure of
the affinity an enzyme
