HW Lab Questions
1. What was the enzyme that was used and why?
The enzyme used was Alkaline phosphatase (ALP) and it was used to because it catalyzes the
hydrolysis of a phosphate group attached to proteins or lipids in the substrate.
2. What created the color change and how?
The hydrolysis the phosphate yields in the substrate para nitrophenol-phosphate (pNPP).
3. Why was the alkaline buffer used for Parts 2 and 3 but not for Part 1?
It was used in parts 2 and 3 to make sure that the pH doesn’t change/
4. Why was it essential to wait to add the enzyme?
It was essential because once you add the enzyme the reaction will start. So if you added it and
let it sit the reaction would've been started and you would be able to see the change in
concentration or absorbance.
5. Collect four peer-reviewed sources on the topic of enzyme properties, the effect of
environmental conditions on enzyme activity, or ALP for the enzyme lab report. Your
response to this question must consist of four appropriate APA-style citations.
Zhang H, Gong L, Liu X, Shao K, Li XZ, Li RX. [Soil Enzyme Activity in Picea schrenkiana
and Its Relationship with Environmental Factors in the Tianshan Mountains, Xinjiang]. Huan
Jing Ke Xue. 2021 Jan 8;42(1):403-410. Chinese. doi: 10.13227/j.hjkx.202004199. PMID:
33372493.
Nakanishi H, Onose S, Kitahara E, Chumchuen S, Takasaki M, Konishi H, Kanekatsu R. Effect
of environmental conditions on the α-glucosidase inhibitory activity of mulberry leaves. Biosci
Biotechnol Biochem. 2011;75(12):2293-6. doi: 10.1271/bbb.110407. Epub 2011 Dec 7. PMID:
22146716.
Zheng YH, Liu J, Ma YZ, Xu Y, Xu F, Hua TC. Temperature effects on enzyme activity of
chicken liver esterase used in calorimetric biosensor. Artif Cells Blood Substit Immobil
Biotechnol. 2012 Feb;40(1-2):125-31. doi: 10.3109/10731199.2011.611470. Epub 2011 Sep 26.
PMID: 21942624.
Phipps J, Chen H, Donovan C, Dominguez D, Morgan S, Weidman B, Fan C, Beyzavi H.
Catalytic Activity, Stability, and Loading Trends of Alcohol Dehydrogenase Enzyme
Encapsulated in a Metal-Organic Framework. ACS Appl Mater Interfaces. 2020 Jun
10;12(23):26084-26094. doi: 10.1021/acsami.0c06964. Epub 2020 Jun 1. PMID: 32478509;
PMCID: PMC7815252.
1. What was the enzyme that was used and why?
The enzyme used was Alkaline phosphatase (ALP) and it was used to because it catalyzes the
hydrolysis of a phosphate group attached to proteins or lipids in the substrate.
2. What created the color change and how?
The hydrolysis the phosphate yields in the substrate para nitrophenol-phosphate (pNPP).
3. Why was the alkaline buffer used for Parts 2 and 3 but not for Part 1?
It was used in parts 2 and 3 to make sure that the pH doesn’t change/
4. Why was it essential to wait to add the enzyme?
It was essential because once you add the enzyme the reaction will start. So if you added it and
let it sit the reaction would've been started and you would be able to see the change in
concentration or absorbance.
5. Collect four peer-reviewed sources on the topic of enzyme properties, the effect of
environmental conditions on enzyme activity, or ALP for the enzyme lab report. Your
response to this question must consist of four appropriate APA-style citations.
Zhang H, Gong L, Liu X, Shao K, Li XZ, Li RX. [Soil Enzyme Activity in Picea schrenkiana
and Its Relationship with Environmental Factors in the Tianshan Mountains, Xinjiang]. Huan
Jing Ke Xue. 2021 Jan 8;42(1):403-410. Chinese. doi: 10.13227/j.hjkx.202004199. PMID:
33372493.
Nakanishi H, Onose S, Kitahara E, Chumchuen S, Takasaki M, Konishi H, Kanekatsu R. Effect
of environmental conditions on the α-glucosidase inhibitory activity of mulberry leaves. Biosci
Biotechnol Biochem. 2011;75(12):2293-6. doi: 10.1271/bbb.110407. Epub 2011 Dec 7. PMID:
22146716.
Zheng YH, Liu J, Ma YZ, Xu Y, Xu F, Hua TC. Temperature effects on enzyme activity of
chicken liver esterase used in calorimetric biosensor. Artif Cells Blood Substit Immobil
Biotechnol. 2012 Feb;40(1-2):125-31. doi: 10.3109/10731199.2011.611470. Epub 2011 Sep 26.
PMID: 21942624.
Phipps J, Chen H, Donovan C, Dominguez D, Morgan S, Weidman B, Fan C, Beyzavi H.
Catalytic Activity, Stability, and Loading Trends of Alcohol Dehydrogenase Enzyme
Encapsulated in a Metal-Organic Framework. ACS Appl Mater Interfaces. 2020 Jun
10;12(23):26084-26094. doi: 10.1021/acsami.0c06964. Epub 2020 Jun 1. PMID: 32478509;
PMCID: PMC7815252.
