PhysioEx Exercise 8 Activity 2 https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex8/act2/
You correctly answered: see what a positive Benedict's test should look like.
2 Which tube is included to detect contaminating amylase in the buffer or in cellulose?
You correctly answered: None of the above.
3 What is the usual substrate for the pancreatic enzyme peptidase?
You correctly answered: peptides.
Experiment Data
Tube No. Reagent 1 Reagent 2 Reagent 3 Time Temp (°C) IKI Benedict's
(min)
pH 7.0
1 Amylase Starch 60 37 - ++
Buffer
pH 7.0
2 Amylase Glucose 60 37 - ++
Buffer
pH 7.0
3 Amylase Cellulose 60 37 - -
Buffer
pH 7.0 Deionized
4 Cellulose 60 37 - -
Buffer Water
pH 7.0
5 Peptidase Starch 60 37 + -
Buffer
6 Bacteria Cellulose pH 7.0 60 37 - ++
Buffer
Post-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.
1 The substrate(s) for amylase is/are
You correctly answered: starch.
2 The results of tube 5 (peptidase, starch, pH 7.0 buffer) demonstrated that
You correctly answered: peptidase does not digest starch.
3 Why was the cellulose in tube 6 (bacteria, cellulose, pH 7.0 buffer) hydrolyzed to glucose?
You correctly answered: The bacterial suspension contained the enzyme cellulase, which
digested the cellulose.
4 Which tube allows you to conclude that the buffer was not contaminated with amylase?
2 of 3 6/16/2021, 11:24 PM
You correctly answered: see what a positive Benedict's test should look like.
2 Which tube is included to detect contaminating amylase in the buffer or in cellulose?
You correctly answered: None of the above.
3 What is the usual substrate for the pancreatic enzyme peptidase?
You correctly answered: peptides.
Experiment Data
Tube No. Reagent 1 Reagent 2 Reagent 3 Time Temp (°C) IKI Benedict's
(min)
pH 7.0
1 Amylase Starch 60 37 - ++
Buffer
pH 7.0
2 Amylase Glucose 60 37 - ++
Buffer
pH 7.0
3 Amylase Cellulose 60 37 - -
Buffer
pH 7.0 Deionized
4 Cellulose 60 37 - -
Buffer Water
pH 7.0
5 Peptidase Starch 60 37 + -
Buffer
6 Bacteria Cellulose pH 7.0 60 37 - ++
Buffer
Post-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.
1 The substrate(s) for amylase is/are
You correctly answered: starch.
2 The results of tube 5 (peptidase, starch, pH 7.0 buffer) demonstrated that
You correctly answered: peptidase does not digest starch.
3 Why was the cellulose in tube 6 (bacteria, cellulose, pH 7.0 buffer) hydrolyzed to glucose?
You correctly answered: The bacterial suspension contained the enzyme cellulase, which
digested the cellulose.
4 Which tube allows you to conclude that the buffer was not contaminated with amylase?
2 of 3 6/16/2021, 11:24 PM
