DNA Cloning and Recombinant DNA Technology Exam Questions and Answers Already Passed
Short fragments - Answers Segments of DNA typically less than 100 bp.
Long fragments - Answers DNA segments longer than 100 bp.
Double strand DNA - Answers DNA consisting of two complementary strands.
Reversible terminators - Answers Nucleotides that can be added and removed.
PCR amplification - Answers Technique to amplify DNA segments exponentially.
Single molecule sequencing - Answers Sequencing method that skips PCR amplification.
Nanopore sequencing - Answers Technique allowing real-time DNA sequencing.
Plasmid - Answers Circular DNA used as a vector in cloning.
Restriction endonucleases - Answers Enzymes that cut DNA at specific sequences.
Genomic libraries - Answers Collections of DNA fragments from an organism.
cDNA libraries - Answers Libraries representing mRNA from samples.
Reverse Transcriptase (RT) - Answers Enzyme converting RNA to complementary DNA.
Microarray analysis - Answers Technique measuring expression of multiple mRNAs.
In situ hybridization - Answers Technique visualizing specific RNA in tissues.
E. coli - Answers Common bacterium used for protein production.
Inducible lac promoter - Answers Promoter activated by lactose for gene expression.
Sticky ends - Answers Single-stranded ends of DNA after restriction cuts.
Transformation - Answers Process of introducing foreign DNA into cells.
DNA libraries - Answers Permanent collections of cloned DNA sequences.
Cloning - Answers Creating identical copies of DNA or cells.
Transcription factors - Answers Proteins regulating gene expression at transcription.
Histone code - Answers Regulatory code based on histone modifications.
Gene expression - Answers Process of converting DNA into functional products.
Recombinant DNA technology - Answers Techniques for combining DNA from different sources.
Short fragments - Answers Segments of DNA typically less than 100 bp.
Long fragments - Answers DNA segments longer than 100 bp.
Double strand DNA - Answers DNA consisting of two complementary strands.
Reversible terminators - Answers Nucleotides that can be added and removed.
PCR amplification - Answers Technique to amplify DNA segments exponentially.
Single molecule sequencing - Answers Sequencing method that skips PCR amplification.
Nanopore sequencing - Answers Technique allowing real-time DNA sequencing.
Plasmid - Answers Circular DNA used as a vector in cloning.
Restriction endonucleases - Answers Enzymes that cut DNA at specific sequences.
Genomic libraries - Answers Collections of DNA fragments from an organism.
cDNA libraries - Answers Libraries representing mRNA from samples.
Reverse Transcriptase (RT) - Answers Enzyme converting RNA to complementary DNA.
Microarray analysis - Answers Technique measuring expression of multiple mRNAs.
In situ hybridization - Answers Technique visualizing specific RNA in tissues.
E. coli - Answers Common bacterium used for protein production.
Inducible lac promoter - Answers Promoter activated by lactose for gene expression.
Sticky ends - Answers Single-stranded ends of DNA after restriction cuts.
Transformation - Answers Process of introducing foreign DNA into cells.
DNA libraries - Answers Permanent collections of cloned DNA sequences.
Cloning - Answers Creating identical copies of DNA or cells.
Transcription factors - Answers Proteins regulating gene expression at transcription.
Histone code - Answers Regulatory code based on histone modifications.
Gene expression - Answers Process of converting DNA into functional products.
Recombinant DNA technology - Answers Techniques for combining DNA from different sources.
