Principles of Recombinant DNA Technology Exam Solutions Manual Rated 100%

Principles of Recombinant DNA Technology Exam Solutions Manual Rated 100% Plasmid - Answers Autonomously replicating circular DNA in bacteria. Bioprocess Engineering - Answers Maintaining sterile conditions for microbial growth. Phenotype - Answers Observable characteristics of an organism. Recombinant DNA - Answers DNA formed by combining DNA from different sources. Gene Cloning - Answers Isolating and replicating a specific gene. Gene Transfer - Answers Introducing a gene into a host organism. Origin of Replication - Answers DNA sequence where replication begins. Restriction Enzymes - Answers Enzymes that cut DNA at specific sequences. Hind I - Answers First restriction enzyme identified, cuts specific sequences. Recognition Sequence - Answers Specific DNA sequence recognized by restriction enzymes. Sticky Ends - Answers Single-stranded DNA ends that can bond with complementary DNA. DNA Ligase - Answers Enzyme that joins DNA fragments together. Gel Electrophoresis - Answers Technique for separating DNA fragments by size. Transformation - Answers Process of introducing foreign DNA into a cell. Antibiotic Resistance Gene - Answers Gene that provides resistance to antibiotics. Eukaryotic Cell - Answers Cell with a nucleus and membrane-bound organelles. Asexual Reproduction - Answers Reproduction without genetic variation. Sexual Reproduction - Answers Reproduction involving genetic variation. Hybridization - Answers Crossbreeding to combine desirable traits. Bacteriophage - Answers Virus that infects bacteria. Palindromic Sequence - Answers DNA sequence reading the same both directions. Exonucleases - Answers Enzymes that remove nucleotides from DNA ends. Endonucleases - Answers Enzymes that cut DNA at specific internal sites. DNA Fragment - Answers Piece of DNA resulting from restriction enzyme action. Genome - Answers Complete set of an organism's DNA. Host Organism - Answers Organism receiving foreign DNA. Cloning - Answers Making multiple identical copies of DNA. Microbial Contamination - Answers Unwanted microbial presence in a culture. Biotechnological Products - Answers Products made using biological processes. DNA Fragments - Answers Negatively charged pieces of DNA used in experiments. Agarose Gel - Answers Natural polymer matrix for DNA separation. Electrophoresis - Answers Technique to separate DNA by size using electric field. Anode - Answers Positive electrode where negatively charged DNA moves. Ethidium Bromide - Answers Staining agent for visualizing DNA under UV light. Elution - Answers Process of extracting DNA from agarose gel. Cloning Vectors - Answers Plasmids or bacteriophages used to replicate DNA. Origin of Replication (ori) - Answers Sequence where DNA replication initiates in vectors. Selectable Marker - Answers Gene allowing identification of transformed cells. Antibiotic Resistance Genes - Answers Genes providing resistance to specific antibiotics. Insertional Inactivation - Answers Disruption of a gene by inserting foreign DNA. B-galactosidase - Answers Enzyme used for colorimetric detection of recombinants. Chromogenic Substrate - Answers Substance producing color change in bacterial colonies. Agrobacterium tumefaciens - Answers Bacterium used to transfer DNA into plants. T-DNA - Answers DNA transferred by Agrobacterium to plant cells. Ti Plasmid - Answers Modified plasmid used for gene delivery in plants. Competent Host - Answers Bacterial cells made capable of taking up DNA. Divalent Cation - Answers Ion like calcium used to induce competence in bacteria. Heat Shock - Answers Temperature change to facilitate DNA uptake in bacteria. Plasmids - Answers Circular DNA molecules used as cloning vectors.