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BOC Study Guide Histotechnology 2nd Ed. - Fixation (HT) Questions and Answers Graded A+

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BOC Study Guide Histotechnology 2nd Ed. - Fixation (HT) Questions and Answers Graded A+ What is the primary purpose of fixation in histotechnology? A) To enhance staining B) To preserve tissue morphology C) To increase tissue size D) To accelerate decomposition Which of the following is a common fixative used in histology? A) Formaldehyde B) Ethanol C) Acetone D) Isopropanol What is the optimal concentration of formaldehyde for fixation? A) 1% B) 5% 2 C) 10% D) 20% What type of fixation preserves lipids better? A) Formalin fixation B) Osmium tetroxide fixation C) Alcohol fixation D) Picric acid fixation What is a disadvantage of using glutaraldehyde as a fixative? A) It can cause excessive cross-linking B) It is less effective than formaldehyde C) It does not penetrate tissues well D) It is non-toxic How long should tissues typically remain in formalin for proper fixation? A) 30 minutes B) 6-24 hours 3 C) 48 hours D) 1 hour What happens to tissue if fixation is inadequate? A) It may result in poor staining quality B) It will be perfectly preserved C) It will shrink significantly D) It will be over-fixed Which of the following is NOT a primary objective of fixation? A) Preventing autolysis B) Enhancing tissue elasticity C) Stabilizing proteins D) Preserving cellular architecture What is the role of co-fixatives in histotechnology? A) To decrease fixation time B) To improve fixation quality

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BOC Study Guide Histotechnology 2nd
Ed. - Fixation (HT) Questions and
Answers Graded A+
What is the primary purpose of fixation in histotechnology?

A) To enhance staining


✔✔B) To preserve tissue morphology


C) To increase tissue size

D) To accelerate decomposition




Which of the following is a common fixative used in histology?


✔✔A) Formaldehyde


B) Ethanol

C) Acetone

D) Isopropanol




What is the optimal concentration of formaldehyde for fixation?

A) 1%

B) 5%


1

,✔✔C) 10%


D) 20%




What type of fixation preserves lipids better?

A) Formalin fixation


✔✔B) Osmium tetroxide fixation


C) Alcohol fixation

D) Picric acid fixation




What is a disadvantage of using glutaraldehyde as a fixative?


✔✔A) It can cause excessive cross-linking


B) It is less effective than formaldehyde

C) It does not penetrate tissues well

D) It is non-toxic




How long should tissues typically remain in formalin for proper fixation?

A) 30 minutes


✔✔B) 6-24 hours


2

, C) 48 hours

D) 1 hour




What happens to tissue if fixation is inadequate?


✔✔A) It may result in poor staining quality


B) It will be perfectly preserved

C) It will shrink significantly

D) It will be over-fixed




Which of the following is NOT a primary objective of fixation?

A) Preventing autolysis


✔✔B) Enhancing tissue elasticity


C) Stabilizing proteins

D) Preserving cellular architecture




What is the role of co-fixatives in histotechnology?

A) To decrease fixation time


✔✔B) To improve fixation quality


3

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