BMSC 230: Module 2 – Glycolysis Questions and
Answers 100% Solved
What are the glucose transporters (5) and where they are found in the body? GLUT1 -
all mammalian tissues
GLUT2 - liver and pancreatic B-cells
GLUT3 - all mammalian tissues
GLUT4 - muscle and fat cells
GLUT5 - small intestine
what is the Km of the 5 glucose transporters? GLUT 1&3 - 1mM
GLUT2 - 15-20mM
GLUT4 - 5mM
GLUT5 - 15mM
What do each of the glucose transporters do? (besides transport glucose) GLUT 1&3 -
Basal glucose uptake
GLUT2 -In the pancreases, it plays a role in the regulation of insulin; in the liver, it removes the
excess glucose from the blood
GLUT4 - Amount in muscle plasma membrane increases with endurance training
GLUT5 - Primarily a fructose transporter
, BMSC 230: Module 2 – Glycolysis Questions and
Answers 100% Solved
What are the normal glucose levels in the blood stream? 5 mM/L when fasting
If two glucose transporters have a Km of 1mM and 15-20mM, which will saturate first? which
is higher affinity? The transporter of 1mM would saturate first and have a higher
affinity as it would reach Vmax faster then the other transporter of 15-20mM.
What are the two main stages of glycolysis? Stage 1. traps glucose in the cell and
modifies it so that it can be cleaved into a pair of phosphorylated 3-carbon compounds [**net
input of energy]
Stage 2. oxidizes the 3-carbon compounds to pyruvate while generating two molecules of ATP
[**net output of energy]
What is step 1 of glycolysis? Phosphorylation of glucose: when glucoe enters the cell, it
is rapidly phosphorylated by hexokinase to form glucose-6-phosphate.
What enzyme is used in step 1 of glycolysis? what does it do? Is it reversable?
Hexokinase - it takes energy from ATP to add a phosphate group onto glucose to make
glucose-6-phosphate
- this is not reversible
Answers 100% Solved
What are the glucose transporters (5) and where they are found in the body? GLUT1 -
all mammalian tissues
GLUT2 - liver and pancreatic B-cells
GLUT3 - all mammalian tissues
GLUT4 - muscle and fat cells
GLUT5 - small intestine
what is the Km of the 5 glucose transporters? GLUT 1&3 - 1mM
GLUT2 - 15-20mM
GLUT4 - 5mM
GLUT5 - 15mM
What do each of the glucose transporters do? (besides transport glucose) GLUT 1&3 -
Basal glucose uptake
GLUT2 -In the pancreases, it plays a role in the regulation of insulin; in the liver, it removes the
excess glucose from the blood
GLUT4 - Amount in muscle plasma membrane increases with endurance training
GLUT5 - Primarily a fructose transporter
, BMSC 230: Module 2 – Glycolysis Questions and
Answers 100% Solved
What are the normal glucose levels in the blood stream? 5 mM/L when fasting
If two glucose transporters have a Km of 1mM and 15-20mM, which will saturate first? which
is higher affinity? The transporter of 1mM would saturate first and have a higher
affinity as it would reach Vmax faster then the other transporter of 15-20mM.
What are the two main stages of glycolysis? Stage 1. traps glucose in the cell and
modifies it so that it can be cleaved into a pair of phosphorylated 3-carbon compounds [**net
input of energy]
Stage 2. oxidizes the 3-carbon compounds to pyruvate while generating two molecules of ATP
[**net output of energy]
What is step 1 of glycolysis? Phosphorylation of glucose: when glucoe enters the cell, it
is rapidly phosphorylated by hexokinase to form glucose-6-phosphate.
What enzyme is used in step 1 of glycolysis? what does it do? Is it reversable?
Hexokinase - it takes energy from ATP to add a phosphate group onto glucose to make
glucose-6-phosphate
- this is not reversible
