BCH EXAM 1
Glycoproteins - correct answer ✔✔carbohydrates bonded to proteins
lipoproteins - correct answer ✔✔lipids attach to protein molecules for transport to the cells to be used
simple proteins - correct answer ✔✔contain only amino acids
prosthetic group - correct answer ✔✔non-protein component of a conjugated protein.
apoprotein - correct answer ✔✔protein without its prosthetic group
holoprotein - correct answer ✔✔protein with prosthetic group
Nucleoproteins - correct answer ✔✔proteins that associate with DNA / nucleic acids
Phosphoproteins - correct answer ✔✔covalently modified by phosphate
metalloproteins - correct answer ✔✔metal ions
Hemoproteins - correct answer ✔✔contain heme groups (iron)
flavoproteins - correct answer ✔✔flavin group, involved in oxidation reduction
isoelectric - correct answer ✔✔least soluble at ___________
salting - correct answer ✔✔process of adding small amounts of NaCl to neutral solution
, makes 2 proteins disguised so they do NOT interact with one another
salting out - correct answer ✔✔(NH4)2SO4 is common for ____________ as you increase ionic strength
there is an increase in proteins coming out of solutions
organic solvents - correct answer ✔✔good for determining solubility in membrane- bound proteins ONLY
electrophoresis - correct answer ✔✔method of separating proteins by electrical charge
1. boil with lipid
2. lipid covers protein
3. protein becomes NEGATIVE
4. can do SDS to see how many there are
SDS - correct answer ✔✔separates proteins based on molecular weight
2D
lighter --> further
below - correct answer ✔✔binds to cation exchange columns _________ pI
eluted by increasing salt concentration or increasing pH
above - correct answer ✔✔binds to anion exchange columns __________ pI
eluted by increasing salt concentration or decreasing pH
Glycoproteins - correct answer ✔✔carbohydrates bonded to proteins
lipoproteins - correct answer ✔✔lipids attach to protein molecules for transport to the cells to be used
simple proteins - correct answer ✔✔contain only amino acids
prosthetic group - correct answer ✔✔non-protein component of a conjugated protein.
apoprotein - correct answer ✔✔protein without its prosthetic group
holoprotein - correct answer ✔✔protein with prosthetic group
Nucleoproteins - correct answer ✔✔proteins that associate with DNA / nucleic acids
Phosphoproteins - correct answer ✔✔covalently modified by phosphate
metalloproteins - correct answer ✔✔metal ions
Hemoproteins - correct answer ✔✔contain heme groups (iron)
flavoproteins - correct answer ✔✔flavin group, involved in oxidation reduction
isoelectric - correct answer ✔✔least soluble at ___________
salting - correct answer ✔✔process of adding small amounts of NaCl to neutral solution
, makes 2 proteins disguised so they do NOT interact with one another
salting out - correct answer ✔✔(NH4)2SO4 is common for ____________ as you increase ionic strength
there is an increase in proteins coming out of solutions
organic solvents - correct answer ✔✔good for determining solubility in membrane- bound proteins ONLY
electrophoresis - correct answer ✔✔method of separating proteins by electrical charge
1. boil with lipid
2. lipid covers protein
3. protein becomes NEGATIVE
4. can do SDS to see how many there are
SDS - correct answer ✔✔separates proteins based on molecular weight
2D
lighter --> further
below - correct answer ✔✔binds to cation exchange columns _________ pI
eluted by increasing salt concentration or increasing pH
above - correct answer ✔✔binds to anion exchange columns __________ pI
eluted by increasing salt concentration or decreasing pH
