Lab 7 Microbial Genetics & Genetic Engineering BIO250L”
Student Name: Kadene Hewit
Access Code (located on the underside of the lid of your lab kit): AC-XTRI809
“Pre-Lab Questions”
1. Which DNA nitrogenous bases pair with each other? Which bases are purines, and which are
pyrimidines?
Adenine and guanine are double-ringed purines and cytosine and thymine
are single ringed pyrimidines
1. How is DNA information used to make proteins? What are the steps of this process?
information contained in the mRNA molecule is translated for amino acids.
2. Give an example of a scenario in which you would perform PCR vs a scenario in which you would
use recombinant DNA technology.
PCR – when you have unknown DNA which is isolated from a specimen in a
small concentration. It can be used for genotyping, cloning, and forensics
3. What occurs during each of the three steps involved in the PCR cycle? How has the use of PCR
changed biotechnology?
1. The denaturation of template into a single strand. 2. Annealing of the
primers for each original new strand. 3. The extension of new DNA strands
from the primer
4. How could you take a protein with a known sequence of amino acids and use it to create an
artificial gene?
Student Name: Kadene Hewit
Access Code (located on the underside of the lid of your lab kit): AC-XTRI809
“Pre-Lab Questions”
1. Which DNA nitrogenous bases pair with each other? Which bases are purines, and which are
pyrimidines?
Adenine and guanine are double-ringed purines and cytosine and thymine
are single ringed pyrimidines
1. How is DNA information used to make proteins? What are the steps of this process?
information contained in the mRNA molecule is translated for amino acids.
2. Give an example of a scenario in which you would perform PCR vs a scenario in which you would
use recombinant DNA technology.
PCR – when you have unknown DNA which is isolated from a specimen in a
small concentration. It can be used for genotyping, cloning, and forensics
3. What occurs during each of the three steps involved in the PCR cycle? How has the use of PCR
changed biotechnology?
1. The denaturation of template into a single strand. 2. Annealing of the
primers for each original new strand. 3. The extension of new DNA strands
from the primer
4. How could you take a protein with a known sequence of amino acids and use it to create an
artificial gene?
