UNE BIOCHEMISTRY MIDTERM STUDY BANK
EXAM LATEST VERSION
What complex in the electron transport chain uses succinate as a substrate?
a.) Complex 1 (NADH-1 oxidoreductase).
b.) Complex IV (Cyt C Oxidase).
c.) Complex V (ATP synthase).
d.) Complex II (Succinate-Q reductase) - Answer Complex II (succinate-Q
reductase) (D)
Which of the following is consistent with an exergonic reaction?
a.) ∆S will be negative.
b.) ∆G will be negative.
c.) ∆S will be positive.
d.) ∆G would be positive, ANSWER ∆G would be
negative (B)
Which of the following characterizes the malate-aspartate shuttle system?
,a.) Transports NADH from the cytoplasm straight to the mitochondria.
b.) Produces 2.5 molecules of ATP in mitochondria per NADH from glycolysis.
c.) Transports NADH from mitochondria to the cytoplasm.
d.) Depends on malate dehydrogenase in the inner mitochondrial membrane.
e.) Requires reducing pyruvate to lactate for oxidizing NADH - ANSWER
Produces 2.5 molecules of ATP in mitochondria per NADH from glycolysis (B)
ATP is a high-energy molecule that is frequently hydrolyzed during reactions.
Which of the following components of ATP stores the majority of its energy?
a.) High-energy phosphate bonds
b.) In the Adenine base
c.) In the D-ribose group - Answer: In the high energy phosphate bonds (A)
Dinitrophenol uncouples the electron transport chain. Which of the following
impacts will be caused by the addition of dinitrophenol?
a.) Lower the pH of the intermembrane gap.
,b.) Increased oxygen consumption.
b.) Reduce oxidation of NADH
d.) Increase ATP generation - ANSWER: Increase oxygen consumption (B).
The malate aspartate shuttle converts glutamate to _______ in the mitochondria
and aspartate to _______ in the cytoplasm.
a.) Oxaloacetate, malate
b.) alpha-ketoglutarate and oxaloacetate
c.) Oxaloacetate and glutamate.
d.) Aspartate and alpha-ketoglutarate
e.) Malate; oxaloacetate - Answer: alpha-ketoglutarate; oxaloacetate (B)
The glycerophosphate shuttle is a key pathway in most tissues. Which of the
following best explains how electrons are transferred by this shuttle?
a.) Electrons are immediately transported from glycerol-3-phosphate to the
NAD-containing glycerol phosphate dehydrogenase
b.) Electrons are directly transported from glycerol-3-phosphate to FAD-
containing glycerol phosphate dehydrogenase.
, c.) Electrons are immediately transported from dihydroxyacetone phosphate
(DHAP) to a FAD-containing glycerol phosphate dehydrogenase.
d.) Electrons are directly transported from glycerol-3-phosphate to FAD, which
contains glycerol phosphate dehydrogenase (B).
In hibernating mammals, brown adipose tissue can create heat. Which of the
following is the most likely process by which this happens?
a.) ATP synthase produces more ATP
b.) Expression of a protein that decouples the ETC.
c.) A higher pH gradient across the inner mitochondrial membrane.
d.) A decrease inoxygen consumption—ANSWER Expression of a protein that
uncouples the ETC (B)
What method would allosteric haemoglobin activators use to boost binding? -
ANSWER: Binding the protein and maintaining its "R" shape.
An enzyme has a mutation in the substate binding site that reduces the binding
of the coenzyme required for covalent catalysis. What are the potential
consequences of this mutation? - ANSWER The enzyme will be unable to
generate the transition state complex.
EXAM LATEST VERSION
What complex in the electron transport chain uses succinate as a substrate?
a.) Complex 1 (NADH-1 oxidoreductase).
b.) Complex IV (Cyt C Oxidase).
c.) Complex V (ATP synthase).
d.) Complex II (Succinate-Q reductase) - Answer Complex II (succinate-Q
reductase) (D)
Which of the following is consistent with an exergonic reaction?
a.) ∆S will be negative.
b.) ∆G will be negative.
c.) ∆S will be positive.
d.) ∆G would be positive, ANSWER ∆G would be
negative (B)
Which of the following characterizes the malate-aspartate shuttle system?
,a.) Transports NADH from the cytoplasm straight to the mitochondria.
b.) Produces 2.5 molecules of ATP in mitochondria per NADH from glycolysis.
c.) Transports NADH from mitochondria to the cytoplasm.
d.) Depends on malate dehydrogenase in the inner mitochondrial membrane.
e.) Requires reducing pyruvate to lactate for oxidizing NADH - ANSWER
Produces 2.5 molecules of ATP in mitochondria per NADH from glycolysis (B)
ATP is a high-energy molecule that is frequently hydrolyzed during reactions.
Which of the following components of ATP stores the majority of its energy?
a.) High-energy phosphate bonds
b.) In the Adenine base
c.) In the D-ribose group - Answer: In the high energy phosphate bonds (A)
Dinitrophenol uncouples the electron transport chain. Which of the following
impacts will be caused by the addition of dinitrophenol?
a.) Lower the pH of the intermembrane gap.
,b.) Increased oxygen consumption.
b.) Reduce oxidation of NADH
d.) Increase ATP generation - ANSWER: Increase oxygen consumption (B).
The malate aspartate shuttle converts glutamate to _______ in the mitochondria
and aspartate to _______ in the cytoplasm.
a.) Oxaloacetate, malate
b.) alpha-ketoglutarate and oxaloacetate
c.) Oxaloacetate and glutamate.
d.) Aspartate and alpha-ketoglutarate
e.) Malate; oxaloacetate - Answer: alpha-ketoglutarate; oxaloacetate (B)
The glycerophosphate shuttle is a key pathway in most tissues. Which of the
following best explains how electrons are transferred by this shuttle?
a.) Electrons are immediately transported from glycerol-3-phosphate to the
NAD-containing glycerol phosphate dehydrogenase
b.) Electrons are directly transported from glycerol-3-phosphate to FAD-
containing glycerol phosphate dehydrogenase.
, c.) Electrons are immediately transported from dihydroxyacetone phosphate
(DHAP) to a FAD-containing glycerol phosphate dehydrogenase.
d.) Electrons are directly transported from glycerol-3-phosphate to FAD, which
contains glycerol phosphate dehydrogenase (B).
In hibernating mammals, brown adipose tissue can create heat. Which of the
following is the most likely process by which this happens?
a.) ATP synthase produces more ATP
b.) Expression of a protein that decouples the ETC.
c.) A higher pH gradient across the inner mitochondrial membrane.
d.) A decrease inoxygen consumption—ANSWER Expression of a protein that
uncouples the ETC (B)
What method would allosteric haemoglobin activators use to boost binding? -
ANSWER: Binding the protein and maintaining its "R" shape.
An enzyme has a mutation in the substate binding site that reduces the binding
of the coenzyme required for covalent catalysis. What are the potential
consequences of this mutation? - ANSWER The enzyme will be unable to
generate the transition state complex.
