CHEM120 OL, Week 7 Lab
OL Lab 13: Protein Synthesis
Learning Objectives
Explain the translation process from mRNA to amino acid
Explain post-translational modification of proteins
Explain protein synthesis processing in the ribosome
Explain the primary, secondary, tertiary and quaternary structures of protein
Explain the basic principles of mass spectrometry (MALDI-TOF)
Introduction
In the Protein Synthesis lab, you will learn about the difference between protein
synthesis in prokaryote (using E. coli) and eukaryote (using Chinese hamster ovarian
(CHO) cells).
Prepare recombinant Erythropoietin and use the mass spectrometer
Your first task in the lab will be to prepare recombinant erythropoietin (EPO) that is
transfected into E. coli and CHO cells. The lab assistant will prepare the recombinant
EPO and you will measure the mass to charge ratio using a mass spectrometer. Not
sure how to handle the mass spectrometer? No worries! You can just take out your
labpad and find an animated video to learn the basics.
Study the translation process from mRNA to amino acids
You will also learn about the translation process from mRNA to amino acids and how
amino acids are assembled to proteins. A 3D animation will describe how codons are
translated into amino acids, how these amino acids are joined together by peptide
bonds creating a primary structure of protein, and how the primary structure is folded
into secondary, tertiary and quaternary structure.
Investigate doping in bike athletes
In the last part of the Protein Synthesis lab, you will use mass spectrometry to
investigate if any athletes are using recombinant human erythropoietin (rhEPO) as a
doping substance. You will do so by collaborating with the doping agent who collects
urine samples at a large bicycle race.
Will you be able to detect if any of the athletes are using doping?
Part 1: Complete Labster Lab: Protein Synthesis
Part 2: Report and Reflection
Purpose: Describe in complete sentences and in your own words, the purpose of this
experiment.
Observations: Record three observations from the simulation.
OL Lab 13: Protein Synthesis
Learning Objectives
Explain the translation process from mRNA to amino acid
Explain post-translational modification of proteins
Explain protein synthesis processing in the ribosome
Explain the primary, secondary, tertiary and quaternary structures of protein
Explain the basic principles of mass spectrometry (MALDI-TOF)
Introduction
In the Protein Synthesis lab, you will learn about the difference between protein
synthesis in prokaryote (using E. coli) and eukaryote (using Chinese hamster ovarian
(CHO) cells).
Prepare recombinant Erythropoietin and use the mass spectrometer
Your first task in the lab will be to prepare recombinant erythropoietin (EPO) that is
transfected into E. coli and CHO cells. The lab assistant will prepare the recombinant
EPO and you will measure the mass to charge ratio using a mass spectrometer. Not
sure how to handle the mass spectrometer? No worries! You can just take out your
labpad and find an animated video to learn the basics.
Study the translation process from mRNA to amino acids
You will also learn about the translation process from mRNA to amino acids and how
amino acids are assembled to proteins. A 3D animation will describe how codons are
translated into amino acids, how these amino acids are joined together by peptide
bonds creating a primary structure of protein, and how the primary structure is folded
into secondary, tertiary and quaternary structure.
Investigate doping in bike athletes
In the last part of the Protein Synthesis lab, you will use mass spectrometry to
investigate if any athletes are using recombinant human erythropoietin (rhEPO) as a
doping substance. You will do so by collaborating with the doping agent who collects
urine samples at a large bicycle race.
Will you be able to detect if any of the athletes are using doping?
Part 1: Complete Labster Lab: Protein Synthesis
Part 2: Report and Reflection
Purpose: Describe in complete sentences and in your own words, the purpose of this
experiment.
Observations: Record three observations from the simulation.
