ascp blood bank certification

. A patient's red cells forward as group O, serum agglutinates B cells (4+) only. Your next step would be: A. Extend reverse typing for 15 minutes B. Perform an antibody screen including a room temperature incubation C. Incubate washed red cells with anti-A1 and anti-A,B for 30 minutes at room temperature D. Test patient's red cells with Dolichos biflorus - correct answersC The strong 4+ reaction in reverse grouping suggests the discrepancy is in forward grouping. Incubating washed red cells at room temperature with anti-A and anti-A,B will enhance reactions. . What reaction would be the same for an A1 and an A2 person? A. Positive reaction with anti-A1 lectin B. Positive reaction with A1 cells C. Equal reaction with anti-H D. Positive reaction with anti-A,B - correct answersD Anti-A,B should react positively with group A or B and any subgroup of A or B (with exception of Am). An A1 (not A2) would react with anti-A1 lectin; only an A2 person with anti-A1 would give a positive reaction with A1 cells; an A2 would react more strongly with anti H than A1 . What should be done if all forward and reverse ABO results as well as the autocontrol are positive? A. Wash the cells with warm saline, autoadsorb the serum at 4°C B. Retype the sample using a different lot number of reagents C. Use polyclonal typing reagents D. Report the sample as group AB Blood bank/Evaluate laboratory and clinical - correct answers. A These results point to a cold autoantibody. Washing the cells with warm saline may elute the autoantibody, allowing a valid forward type to be performed. The serum should be adsorbed using washed cells until the autocontrol is negative. Then the adsorbed serum should be used for reverse typing