Test 1: Bio 1401
BIO 1401 MIDTERM ACTUAL EXAM (2024/2025 UPDATED &
VERIFIED ) COMPLETE Q&A 100% PASS
1. I am using the oil immersion technique in lab what is the total magnification? (you
should know this but the ocular is 10x and the objective is 100x)
a. 1000x
b. 110x
c. 100x
d. 200x
2. The main unit used in this class of measurement is a and is a factor of
10 - ?
a. Micrometer (micron); 10-6
b. Micrometer (micron); 10-9
c. Millimeter; 10-3
d. Centimeter; 10-2
3. What is the purpose of the oil used in the oil immersion technique?
a. Improves the magnification
b. Prevents refraction of light in the air
c. Improves resolving power.
d. A and C
4. If you want to see a ribosome in a eukaryotic cell which microscope would you use?
a. Scanning Electron Microscope (SEM)
b. Transmission Electron Microscope (TEM)
c. A Light Microscope
d. Magnifying Glass
5. During your lab unknowns you will be executing a gram stain. Which tool will you use to
analyze this?
a. The naked eye, because it’s just a color difference.
b. A light microscope
c. Scanning Electron Microscope
d. Transmission Electron Microscope.
6. What role does iodine play in gram staining?
a. Mordant
b. Primary Stain
c. Secondary Stain
d. Decolorizing agent
7. A the H and N spikes used to identify the flu virus is found on the surface of the
microbe, which microscope would your use to see these spikes?
a. Scanning Electron Microscope
b. Transmission Electron Microscope
c. Light Microscope
d. Magnifying glass.
e. B and C
,Test 1: Bio 1401
8. Organisms being viewed under an electron microscope take less time to prepare than
organisms prepared for light microscopes.
a. True
b. False
9. A basic dye have positive charge and dyes the , and an acidic dye has a
negative charge and dyes the .
a. Organism; background
b. Gram (+) organisms; Gram (-) organisms.
c. Sporeformers; Non-sporeformers
d. Acid-Fast; Non-Acid-Fast
10. Which of the following organisms is Acid Fast?
a. Mycobacterium tuberculosis
b. Bacillus anthracis
c. Clostridium tetanii
d. Serratia marcescens
11. If your friend Frodo was trying to gram stain a mysterious bacteria found in middle earth
that he suspects to be Gram (-) , but forgot the most important step. What color would
the bacteria appear.
a. Pink
b. Purple
c. Colorless
d. All the colors of the rainbow cuz its middle earth.
12. T/F. An electron microscope will produce vivid color images.
13. Transmission Electron Microscopes commonly use what in order to produce an image.
a. Gold
b. Osmium
c. Iodine
d. Safranin
14. This Acid-Fast Organism can be grown in Armadillos.
a. Mycobacterium tuberculosis
b. Mycobacterium leprae
c. Bacillus anthracis
d. Serratia marcescens
, Test 1: Bio 1401
Chapter 4
15. Where would 70s ribosomes be used to synthesize proteins? Circle all that apply.
a. Mitochondria
b. Chloroplast
c. Streptococcus aureus
d. Muscle Fibers (cells)
e. All of the above
16. A particular organism found in the ocean dives deep during the day time and rises to the
surface during the night. This type of movement could be classified as
.
a. Positive Chemeotaxis
b. Negative Chemeotaxis
c. Positive Phototaxis
d. Negative Phototaxis.
17. I cause endotoxins. I am only found in the cell walls of bacteria that turn pink when
gram stained. What am I?
a. Lipid A
b. O-polysaccharide.
c. Peptidoglycan
d. Lipoteichoic Acid.
18. Bacterial cell walls are composed of this, which are made up of NAG and NAM units.
a. Peptidoglycan
b. Cellulose
c. Peripheral proteins
d. Phospholipid bilayer
19. What is the morphology of Staphylococcus aureus ?
a. Gram (+) bacilli in a Random arrangement
b. Gram(-) cocci in a Clustered “grape” like arrangement.
c. Gram (+) cocci in a Clustered “grape” like arrangement.
d. Gram (+) cocci in a “chain” like arrangement.
20. Evidence of the endosymbiotic Theory includes
a. Chloroplast and Mitochondria divide by Binary Fission
b. Chloroplasts and Mitochondria contain their own DNA
c. Chloroplasts and Mitochondria contain 80s ribosomes.
d. A and B
e. All of the above.
BIO 1401 MIDTERM ACTUAL EXAM (2024/2025 UPDATED &
VERIFIED ) COMPLETE Q&A 100% PASS
1. I am using the oil immersion technique in lab what is the total magnification? (you
should know this but the ocular is 10x and the objective is 100x)
a. 1000x
b. 110x
c. 100x
d. 200x
2. The main unit used in this class of measurement is a and is a factor of
10 - ?
a. Micrometer (micron); 10-6
b. Micrometer (micron); 10-9
c. Millimeter; 10-3
d. Centimeter; 10-2
3. What is the purpose of the oil used in the oil immersion technique?
a. Improves the magnification
b. Prevents refraction of light in the air
c. Improves resolving power.
d. A and C
4. If you want to see a ribosome in a eukaryotic cell which microscope would you use?
a. Scanning Electron Microscope (SEM)
b. Transmission Electron Microscope (TEM)
c. A Light Microscope
d. Magnifying Glass
5. During your lab unknowns you will be executing a gram stain. Which tool will you use to
analyze this?
a. The naked eye, because it’s just a color difference.
b. A light microscope
c. Scanning Electron Microscope
d. Transmission Electron Microscope.
6. What role does iodine play in gram staining?
a. Mordant
b. Primary Stain
c. Secondary Stain
d. Decolorizing agent
7. A the H and N spikes used to identify the flu virus is found on the surface of the
microbe, which microscope would your use to see these spikes?
a. Scanning Electron Microscope
b. Transmission Electron Microscope
c. Light Microscope
d. Magnifying glass.
e. B and C
,Test 1: Bio 1401
8. Organisms being viewed under an electron microscope take less time to prepare than
organisms prepared for light microscopes.
a. True
b. False
9. A basic dye have positive charge and dyes the , and an acidic dye has a
negative charge and dyes the .
a. Organism; background
b. Gram (+) organisms; Gram (-) organisms.
c. Sporeformers; Non-sporeformers
d. Acid-Fast; Non-Acid-Fast
10. Which of the following organisms is Acid Fast?
a. Mycobacterium tuberculosis
b. Bacillus anthracis
c. Clostridium tetanii
d. Serratia marcescens
11. If your friend Frodo was trying to gram stain a mysterious bacteria found in middle earth
that he suspects to be Gram (-) , but forgot the most important step. What color would
the bacteria appear.
a. Pink
b. Purple
c. Colorless
d. All the colors of the rainbow cuz its middle earth.
12. T/F. An electron microscope will produce vivid color images.
13. Transmission Electron Microscopes commonly use what in order to produce an image.
a. Gold
b. Osmium
c. Iodine
d. Safranin
14. This Acid-Fast Organism can be grown in Armadillos.
a. Mycobacterium tuberculosis
b. Mycobacterium leprae
c. Bacillus anthracis
d. Serratia marcescens
, Test 1: Bio 1401
Chapter 4
15. Where would 70s ribosomes be used to synthesize proteins? Circle all that apply.
a. Mitochondria
b. Chloroplast
c. Streptococcus aureus
d. Muscle Fibers (cells)
e. All of the above
16. A particular organism found in the ocean dives deep during the day time and rises to the
surface during the night. This type of movement could be classified as
.
a. Positive Chemeotaxis
b. Negative Chemeotaxis
c. Positive Phototaxis
d. Negative Phototaxis.
17. I cause endotoxins. I am only found in the cell walls of bacteria that turn pink when
gram stained. What am I?
a. Lipid A
b. O-polysaccharide.
c. Peptidoglycan
d. Lipoteichoic Acid.
18. Bacterial cell walls are composed of this, which are made up of NAG and NAM units.
a. Peptidoglycan
b. Cellulose
c. Peripheral proteins
d. Phospholipid bilayer
19. What is the morphology of Staphylococcus aureus ?
a. Gram (+) bacilli in a Random arrangement
b. Gram(-) cocci in a Clustered “grape” like arrangement.
c. Gram (+) cocci in a Clustered “grape” like arrangement.
d. Gram (+) cocci in a “chain” like arrangement.
20. Evidence of the endosymbiotic Theory includes
a. Chloroplast and Mitochondria divide by Binary Fission
b. Chloroplasts and Mitochondria contain their own DNA
c. Chloroplasts and Mitochondria contain 80s ribosomes.
d. A and B
e. All of the above.
