CMB1003 L3 Exam With Complete Solutions
2024-2025
Growth- Answer increase in number of cells Generation time- Answer time required for
one cell to divide to produce 2 cells
Under optimal conditions, what kind of growth does bacteria undergo - Answer
exponential growth
Lag phase- Answer Cells adapt to new environment, synthesize necessary metabolic
enzymes/metabolites
Exponential phase- Answer Optimal growth with consistent doubling in cell numbers
lag phase- the rate at which new biomass is being formed exceeds the rate at which
biomass is being removed
stationary phase- Answer growth is limited by nutrient depletion and accumulation of
toxic metabolites so that the rate of new cell production and rate of cell death is
balanced
death phase- Answer complex, gradual loss of viability, but with some turnover of the
cell population
Measurement of bacterial growth- Answer -plating methods
-turbidity
-direct microscopic counting
-flow cytometry
total cell count- Answer -the total number of bacterial cells
viable cell count- Answer -the number of living bacterial cells
2024-2025
Growth- Answer increase in number of cells Generation time- Answer time required for
one cell to divide to produce 2 cells
Under optimal conditions, what kind of growth does bacteria undergo - Answer
exponential growth
Lag phase- Answer Cells adapt to new environment, synthesize necessary metabolic
enzymes/metabolites
Exponential phase- Answer Optimal growth with consistent doubling in cell numbers
lag phase- the rate at which new biomass is being formed exceeds the rate at which
biomass is being removed
stationary phase- Answer growth is limited by nutrient depletion and accumulation of
toxic metabolites so that the rate of new cell production and rate of cell death is
balanced
death phase- Answer complex, gradual loss of viability, but with some turnover of the
cell population
Measurement of bacterial growth- Answer -plating methods
-turbidity
-direct microscopic counting
-flow cytometry
total cell count- Answer -the total number of bacterial cells
viable cell count- Answer -the number of living bacterial cells
