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BIOD 171 Module 3 Quiz with Verified Answers |Latest 2024/2025

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What is different about phase contrast microscopy compared to brightfield? You are able to visualize things that would otherwise be invisible; cells are highly distinguishable True or False: A cell that is adherent, flat (thin), and unstained is easily identified using bright field microscopy False: Adherent, flat cells are almost invisible due to the limits on both resolution and contrast True or False: The distinguishing characteristic of Gram-Negative bacteria is the presence of LPS in the outer membrane. True True or False: Following the decolorization step of the Gram stain, Gram-Negative bacteria will appear colorless. True True or False: If you wish to study the motiity of an organism you cannot heat fix, but you can chemically fix the specimen False What type of microscopy uses lasers? Confocal Does electron microscopy use live or dead samples? Dead Does electron microscopy use fixation? Yes What type of microscopy are samples coated with gold or platinum? Electron- SEM

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BIOD 171 Module 3 Quiz with Verified
Answers.

What is different about phase contrast microscopy compared to
_;- _;- _;- _;- _;- _;- _;- _;- _;-



brightfield? You are able to visualize things that would
_;-_;- _;- _;- _;- _;- _;- _;- _;- _;- _;-



otherwise be invisible; cells are highly distinguishable
_;- _;- _;- _;- _;- _;-




True or False: A cell that is adherent, flat (thin), and unstained is
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-



easily identified using bright field microscopy
_;- False: _;- _;- _;- _;- _;-_;- _;- _;-



Adherent, flat cells are almost invisible due to the limits on both
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-



resolution and contrast _;- _;-




True or False: The distinguishing characteristic of Gram-Negative
_;- _;- _;- _;- _;- _;- _;- _;-



bacteria is the presence of LPS in the outer membrane.
_;- _;- True
_;- _;- _;- _;- _;- _;- _;- _;-_;- _;-




True or False: Following the decolorization step of the Gram
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;-



stain, Gram-Negative bacteria will appear colorless.
_;- True _;- _;- _;- _;- _;-_;- _;-




True or False: If you wish to study the motiity of an organism
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-



you cannot heat fix, but you can chemically fix the specimen
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-_;-



False
_;-

, What type of microscopy uses lasers?
_;- _;- _;- _;- _;- _;-_;- _;- Confocal


Does electron microscopy use live or dead samples?
_;- _;- _;- _;- _;- _;- _;- _;-_;- _;- Dead


Does electron microscopy use fixation?
_;- _;- _;- _;- _;-_;- _;- Yes


What type of microscopy are samples coated with gold or
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;-



platinum? Electron- SEM _;-_;- _;- _;-




Contrast _;-_;- _;- difference in light absorbance between 2 areas
_;- _;- _;- _;- _;- _;-




Dark field microscopy light
_;- _;- _;- _;-_;- _;- reflects light off of specimen at_;- _;- _;- _;- _;- _;-



an angle
_;-




Can you see intracellular structures with a dark field
_;- _;- _;- _;- _;- _;- _;- _;- _;-



microscope? No _;-_;- _;-




What microscope can capture in 3-D?
_;- _;- _;- _;- _;- _;-_;- _;- Confocal

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