Answers.
What is different about phase contrast microscopy compared to
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brightfield? You are able to visualize things that would
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otherwise be invisible; cells are highly distinguishable
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True or False: A cell that is adherent, flat (thin), and unstained is
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easily identified using bright field microscopy
_;- False: _;- _;- _;- _;- _;-_;- _;- _;-
Adherent, flat cells are almost invisible due to the limits on both
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resolution and contrast _;- _;-
True or False: The distinguishing characteristic of Gram-Negative
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bacteria is the presence of LPS in the outer membrane.
_;- _;- True
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True or False: Following the decolorization step of the Gram
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stain, Gram-Negative bacteria will appear colorless.
_;- True _;- _;- _;- _;- _;-_;- _;-
True or False: If you wish to study the motiity of an organism
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you cannot heat fix, but you can chemically fix the specimen
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False
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, What type of microscopy uses lasers?
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Does electron microscopy use live or dead samples?
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Does electron microscopy use fixation?
_;- _;- _;- _;- _;-_;- _;- Yes
What type of microscopy are samples coated with gold or
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platinum? Electron- SEM _;-_;- _;- _;-
Contrast _;-_;- _;- difference in light absorbance between 2 areas
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Dark field microscopy light
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an angle
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Can you see intracellular structures with a dark field
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microscope? No _;-_;- _;-
What microscope can capture in 3-D?
_;- _;- _;- _;- _;- _;-_;- _;- Confocal