mip 302 practical exam study guide

mip 302 practical exam study guide colony shapes - Answer️️ -Circular, irregular, rhizoid (spider), punctiform (stipple) colony textures - Answer️️ -rough, dry, wrinkled, shiny, dull colony margins - Answer️️ -entire, erose, filamentous, lobate colony colors - Answer️️ -translucent, yellow, white, cream, etc what should be included when asked to characterize a colony? - Answer️️ -colony shape, texture, margin, and color Why is broth not used as an isolation medium? - Answer️️ -Can't differentiate between types of bacteria should TSA slant be used as an isolation medium? - Answer️️ -probably not - the surface area is generally too small to differentiate between types of bacteria What color do gram positive bacteria stain? - Answer️️ -purple What color do gram negative bacteria stain? - Answer️️ -pink Why do gram positive bacteria stain the color they do? - Answer️️ -g + bacteria stain purple because of their thick cell wall that contains peptidoglycan and teichoic acids In the gram stain, what is the primary stain? - Answer️️ -crystal violet what color do G+ and G- stain after the primary stain ONLY? - Answer️️ -purple ©SOPHIABENNETT EXAM SOLUTIONS Saturday, September 7, 2024 6:58 PM Page | 2 in a gram stain, what is gram's iodine used for? - Answer️️ -gram's iodine is a mordant used to help the crystal violet (primary stain) properly bind to the cell wall so it is not easily washed out of G+ cells in the gram stain, what is the purpose of decolorizer? - Answer️️ -To selectively remove stain from cells what would happen if you forgot gram's iodine in a gram stain? - Answer️️ -G+ cells and G- cells would stain pink because the primary stain couldn't fully bind to the G+ cell wall in the gram stain, what is the counterstain and its purpose? - Answer️️ -safranin turns G- cells pink/red and allows the G- cells to be visualized what would happen if you forgot to counterstain in a gram stain? - Answer️️ -G+ cells would stain purple while G- cells would remain colorless types of differential stains - Answer️️ -gram stain, acid-fast stain, endospore stain what does gram staining differentiate between and how? - Answer️️ -gram staining differentiates between gram positive and gram negative bacteria based on the composition of bacterial cell walls what does acid-fast staining differentiate between and how? - Answer️️ -acid-fast staining differentiates between acid-fast positive and negative cells