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MIP 300 Final exam with Answers to Questions

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MIP 300 Final exam with Answers to Questions

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MIP 300
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Institution
MIP 300
Course
MIP 300

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Uploaded on
September 5, 2024
Number of pages
29
Written in
2024/2025
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Exam (elaborations)
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Questions & answers

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MIP 300 Final exam with Answers to Questions

the organism with the more positive reduction potential will produce more energy -
✔✔true



dna ligase forms - ✔✔the last phosphodiester bond between dna single strands



dna ligase forms hydrogen bonds - ✔✔false


for each acetyl-coA that enters the TCA cycle, how many ATP are generayed by
substrate level phosphorylation - ✔✔1


you correctly perform a gran stain on bacteria that contain LPS, what color will you see
on the slide? - ✔✔pink


a bacterium contains 2 carbohydrates and 5 different amino acids in its cell wall. What
color will it gram stain? - ✔✔purple


an antibiotic inhibits ribosome function and prevents bacteria from sporulating. which
step in sporulation is affected - ✔✔formation of spore coats


a bacterium is known to be a psychophile. which of the following would be true for this
organism? - ✔✔generation time shortest at 0-20 degrees celcsius and growth rate
the fastest


you grow a bacteria in a tube that is loosely capped. it grows evenly throughout the
tube. the bacteria is likely - ✔✔aerotolerant anaerobe

,a bacterium has recently acquired antibiotic resistance, which of the following could
NOT be a way this occurred - ✔✔someone stopped taking her antibiotics early


which of the following antibiotic mechanisms would be achieve selective toxicity -
✔✔stop cell wall synthesis


what is the most effective type of vaccine? will you have full immunity if you get the
vaccine 5 days before exposure - ✔✔attenuated; no


you have abdominal pain, headache and a bacterial blood infection. are these signs or
symptoms? what type of antibody? - ✔✔symptoms and IgG


you isolate a new canine virus that is similiar to a virus previously seen in birds. what
type of change has occured? - ✔✔antigenic shift


newborns get some protections from their mothers. what is protecting them? where is
the protection coming from? - ✔✔IgA and igG and breast milk and placenta


how many different B cells will be specific for a bacterium with a protein with 4 binding
sites and a protein with 3 - ✔✔7, one B cell for each epitope


covid-19 appears to be transmitted before someone develops signs or symptoms. what
type of carrier is this? - ✔✔incubatory carrier


you go to the ER for a severe drop in blood pressure. which of the following might be
responsible? how will it stain? - ✔✔endotoxin or superantigen, pink or purple

, a patient has a B cell mutation that prevents antibody production. Which can they still
do at normal levels? - ✔✔activate CTLs specific for the virus


you start having diarrhea 4 hours after eating at a sketchy taco shop. This is likely a -
✔✔food intoxication


to ensure a gene inserted in a plasmid is in the correct orientation you should use -
✔✔2 different sticky ends



prions cause disease by - ✔✔converting normal proteins to abnormal proteins


an antiviral that inactivates transcriptase activity will be effective against a + sense RNA
virus - ✔✔false


for a negative sense RNA virus, protein translation will start before replicase acts -
✔✔true


you get a cold sore which heals then you get another one 2 years later. what likely
causes this? - ✔✔the virus was latent during this time


you mix dead S strain with a live R strain of bacteria that is not competent. Will the
mouse live or die? - ✔✔mouse will live


a bacteria took up pUC19 that did not contain the gene of interest. what will be on your
plate with ampicillin - ✔✔the colonies will be blue


what do you observe if you forgot to add the fluorescent labeled antibody in an ELISA? -
✔✔potential false negative

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