ALL ANSWERS VERIFIED
What does the D-value represent? - Answer-The time in minutes required to kill 90% of
microbial population.
What does the Z-value represent? - Answer-The increase in temp required to reduce
microbes by one log.
What factors affect the effectiveness of antimicrobial agents? - Answer--pop. size
-pop. composition or susceptibility
-conc. and intensity of agents
-duration of exposure
-local environment
-temp
What is pasteurization? - Answer--heat at 55-60ºC for 30 mins
-Flash pasteurize at 72ºC for 15 secs
-ultra high temp pasteurization at 150ºC for 1-3 secs
-not considered sterilization
What technique kills everything, including spores? - Answer-Autoclaving
What is filtration? - Answer-A type of sterilization but does not filter out viruses.
Laminar Flow Biological Safety Cabinent - Answer--Contains high-efficiency particulate
air filters
-Used when working with high-risk microbes or experiments requiring high level of
sterility
What is a disinfectants? - Answer-Can be used on non-living surfaces.
What is a antiseptics? - Answer-Can be applied to living.
What do detergents do? - Answer-break down membrane and denature proteins
Ethylene oxide - Answer-used in sterilizer to sterilize various objects
Phenols - Answer--included in many antibacterial hand soaps, toothpastes, and more
-turns on multi-drug resistance
-contain carcinogens