MOLECULAR BIOLOGY ASCP PRACTICE QUESTIONS
2024
This refers to a means by which large quantities of DNA can be amplified from a small
amount of DNA template:
A. PCR
B. DNA mass production
C. DNA synthesis
D. DNA birth - Answers -PCR
With regards to PCR, which of the following is NOT accurate?
A. Denature strands at 94 degrees
B. Base pairing of primers at 60 degrees
C. Elongation of primers using Taq polymerase at 72 degrees
D. Filtration of DNA at 40 degrees - Answers -Filtration of DNA at 40 degrees
Which of the following PCR techniques is the best one for detecting point mutation in
DNA?
A. RT PCR
B. PCR
C. Allele-specific PCR
D. Anchored PCR - Answers -Allele-Specific PCR
What happens after many cycles of PCR?
A. Exponential Amplification
B. Diminished Amplification
C. Product lysing
D. DNA translation - Answers -Exponential Amplification
Which of the following is NOT true about the Taq DNA polymerase?
A. It allows for the ability to use PCR to automate the process of amplification
B. It can be heat resistant
C. It is ubiquitous in nature except for the hot springs
D. It is most efficient at a certain optimal temperature. - Answers -C
The PCR technique used to specifically amplify only one strand of the target DNA by
using only one unequal primer concentration is:
A. qPCR
B. Asymmetric PCR
C. Anchored PCR
D. Nested PCR - Answers -Asymmetric PCR
Sarah wants to turn up the heat on her heat block to thaw her DNA samples at over 100
degrees for an hour. What is the likely result of her PCR?
A. She will get much more amplified DNA product
, B. She will not get a significant amplified DNA product
C. She will only get minimal synthesis
D. She will likely not get any synthesis - Answers -She will likely not get any synthesis
When setting up her thermocycler, Sarah wants to anneal her DNA strands at a
temperature much higher than 60 degrees. What is the likely result of her action?
A. The DNA strands will likely not anneal as desired.
B. The DNA strands will likely anneal more optimally.
C. The DNA strands will anneal, and then form a triple helix.
D. The DNA strands will anneal but will not form a double helix. - Answers -The DNA
strands will likely not anneal as desired.
The process of determining differences in an individual's genotype by comparing one's
DNA sequence to another individual's sequence or a reference. It shows the possible
alleles that a person has inherited from their parents. Which of the following does the
statement above refer to?
A. Genetics of curiosity
B. Genotyping
C. Gene Modification
D. Phenotyping - Answers -Genotyping
The PCR procedure, where Taq polymerase is added after the rest of the PCR
components are heated to the DNA melting temperature, so as to avoid non-specific
amplification at lower temperatures is:
A. Asymmetric PCR
B. Hotstart PCR
C. Multiplex PCR
D. Nested PCR - Answers -Hotstart PCR
Which of the following is NOT true about HCV Genotypes?
A. The genotype determines treatment protocol
B. HCV 1 is most common and most difficult to treat
C. HCV 1 is associated with a less severe liver disease than 2 and 3
D. HCV 2 and 3 are three times more likely than 1 to respond to therapy with alpha-INF
or combo with riboflavin - Answers -HCV 1 is associated with a less severe liver disease
than 2 and 3
Which of the following syndromes do these statements refer to?
I: results from severe reduction of FMRP protein encoded by FMR1 gene
II: caused by an unstable repeat of CGG in exon 1
III: genetic disorder leads to the expansion of repeats with aberrant hypermethylation of
CpG
A. Angelman syndrome
B. Fragile X syndrome
C. Prader Willi syndrome
2024
This refers to a means by which large quantities of DNA can be amplified from a small
amount of DNA template:
A. PCR
B. DNA mass production
C. DNA synthesis
D. DNA birth - Answers -PCR
With regards to PCR, which of the following is NOT accurate?
A. Denature strands at 94 degrees
B. Base pairing of primers at 60 degrees
C. Elongation of primers using Taq polymerase at 72 degrees
D. Filtration of DNA at 40 degrees - Answers -Filtration of DNA at 40 degrees
Which of the following PCR techniques is the best one for detecting point mutation in
DNA?
A. RT PCR
B. PCR
C. Allele-specific PCR
D. Anchored PCR - Answers -Allele-Specific PCR
What happens after many cycles of PCR?
A. Exponential Amplification
B. Diminished Amplification
C. Product lysing
D. DNA translation - Answers -Exponential Amplification
Which of the following is NOT true about the Taq DNA polymerase?
A. It allows for the ability to use PCR to automate the process of amplification
B. It can be heat resistant
C. It is ubiquitous in nature except for the hot springs
D. It is most efficient at a certain optimal temperature. - Answers -C
The PCR technique used to specifically amplify only one strand of the target DNA by
using only one unequal primer concentration is:
A. qPCR
B. Asymmetric PCR
C. Anchored PCR
D. Nested PCR - Answers -Asymmetric PCR
Sarah wants to turn up the heat on her heat block to thaw her DNA samples at over 100
degrees for an hour. What is the likely result of her PCR?
A. She will get much more amplified DNA product
, B. She will not get a significant amplified DNA product
C. She will only get minimal synthesis
D. She will likely not get any synthesis - Answers -She will likely not get any synthesis
When setting up her thermocycler, Sarah wants to anneal her DNA strands at a
temperature much higher than 60 degrees. What is the likely result of her action?
A. The DNA strands will likely not anneal as desired.
B. The DNA strands will likely anneal more optimally.
C. The DNA strands will anneal, and then form a triple helix.
D. The DNA strands will anneal but will not form a double helix. - Answers -The DNA
strands will likely not anneal as desired.
The process of determining differences in an individual's genotype by comparing one's
DNA sequence to another individual's sequence or a reference. It shows the possible
alleles that a person has inherited from their parents. Which of the following does the
statement above refer to?
A. Genetics of curiosity
B. Genotyping
C. Gene Modification
D. Phenotyping - Answers -Genotyping
The PCR procedure, where Taq polymerase is added after the rest of the PCR
components are heated to the DNA melting temperature, so as to avoid non-specific
amplification at lower temperatures is:
A. Asymmetric PCR
B. Hotstart PCR
C. Multiplex PCR
D. Nested PCR - Answers -Hotstart PCR
Which of the following is NOT true about HCV Genotypes?
A. The genotype determines treatment protocol
B. HCV 1 is most common and most difficult to treat
C. HCV 1 is associated with a less severe liver disease than 2 and 3
D. HCV 2 and 3 are three times more likely than 1 to respond to therapy with alpha-INF
or combo with riboflavin - Answers -HCV 1 is associated with a less severe liver disease
than 2 and 3
Which of the following syndromes do these statements refer to?
I: results from severe reduction of FMRP protein encoded by FMR1 gene
II: caused by an unstable repeat of CGG in exon 1
III: genetic disorder leads to the expansion of repeats with aberrant hypermethylation of
CpG
A. Angelman syndrome
B. Fragile X syndrome
C. Prader Willi syndrome
