BTEC EXAM REVIEW TEST QUESTIONS
& ANSWERS 2024/2025
What does apply to bacteriophages? - ANSWERSBacteriophages recognize molecules on bacterial surface
to infect
Most phages have narrow host range
Bacteriophage genomes can be RNA, but mostly DNA
All phages use the hosts replication machinery to some extent
In vitro transcription of the SARS-2 spike gene makes use of the __________ - ANSWERST7 gp10
promoter
The antirestriction protein of T7: - ANSWERSprotects T7 DNA from bacterial endonucleases
What is the enzymatic function of gene product 1 in T7? - ANSWERSmRNA synthesis
Which phage-encoded protein catalyzes the formation of phosphodiester bonds between nucleotides? -
ANSWERSDNA ligase
In the pET system for recombinant protein expression, in the absence of IPTG: - ANSWERSBinding of T7
RNA polymerase to the gp10 promoter is obstructed
Which gene or feature is not employed in the pET system? - ANSWERST7 DNA polymerase
What is a major advantage of bacteriophages as antimicrobial agents over small molecule antibiotics? -
ANSWERSless killing of friendly human bacteria
& ANSWERS 2024/2025
What does apply to bacteriophages? - ANSWERSBacteriophages recognize molecules on bacterial surface
to infect
Most phages have narrow host range
Bacteriophage genomes can be RNA, but mostly DNA
All phages use the hosts replication machinery to some extent
In vitro transcription of the SARS-2 spike gene makes use of the __________ - ANSWERST7 gp10
promoter
The antirestriction protein of T7: - ANSWERSprotects T7 DNA from bacterial endonucleases
What is the enzymatic function of gene product 1 in T7? - ANSWERSmRNA synthesis
Which phage-encoded protein catalyzes the formation of phosphodiester bonds between nucleotides? -
ANSWERSDNA ligase
In the pET system for recombinant protein expression, in the absence of IPTG: - ANSWERSBinding of T7
RNA polymerase to the gp10 promoter is obstructed
Which gene or feature is not employed in the pET system? - ANSWERST7 DNA polymerase
What is a major advantage of bacteriophages as antimicrobial agents over small molecule antibiotics? -
ANSWERSless killing of friendly human bacteria
