PURIFICATION of
CoMpQUDS
pOPIfICATIOM
-lo Se peraba
ImpuYitie
SUdinable orqanic cowpoun
Cam phor.
bexachloro naphnqloneanacene,antraquinone,
onzoie aid,
eane
( ) apvsTALLIGATl0
-A most general me oa t
- |4
poify ooid orqanic com pound
USine a Solvont "1n hich toe eom pound 1s paiincghy
Solble at tempoature but a pvecia bly dubte t bcn
4empat r
e eampund grcadualy Cwystallsa t
from leavina b bind all ne
u) DisWLLATGnN
Ponf Loklqle organic compound TUquid)
typen
(a) Gmple dsillation:
Se perale uolalile CompoUnd tom nen \lobile imponttey
Sepeate too misible iqvid i t consdeae ufîerencQ
bolina pont (>2 6c)
" cslo o fom C6c) aniune Cbp-lB4C)
dueyl ehcy (35O u Toluene ( l l ° )
mDtue
teront te mpe qtey.
e t o uaic la po|se a t
Pindple
bolin poln Wauid \apouI Se
fist Te a r s are
coleetd
Ondensec
(b) fractonal Disilla|in
wusscible Liaui
To Gepeyate two
elone
pe to leum\nity
hati ons
Pansed
e Vapuu
Sas temparcat
clunn
, hng eslumn ol multple su face
(colmn)
cc ) acuv m
Diillatn (Drtllatlon ond a preUre ):
oY
made u bol at loe empaetre boy
Phnciple; A s exkmal pre ssue derrere
(d) Steam istilla thon:
-to arl stea Wol atile and ond 'imide
wit a ey
, o no- nito phenl Sal caldehucle 6rho hydoy aetprn
Tbe iaui naving o-\S
callec steam Molaile Liq
at 100C
S4eam vog he bated '1mpure Sam ple ofstea
Volatile poundl slapouy of stean Valatk
(iqvid wlll
pnncsle A always bals a
The nGrnal
a
components
Condiion wwales volatl Pat.
loecawSe
(i) PIFERENWAL TXTRACI0N
- Done by aqveo medOm
rmxtu
) tc solent wwst be
IwwrsCble wnate.
nan
, " chloro oricnloromnehane
Ae more cdenso an atei
Jater mcible soluont al cannol be sed,, foy Aferontal ox traos
e hanol Calcohols), acetoe, diehel etes
(V) CHPOMAT0aPAPHY
- A Veny gonerGl me hod
Mus moden me thsd
miIte
phase is alowed to moe slouly t n he
Staoney nae 3Yaduly 3t
Seperaleel ver e statñonv hoye Cau Sa
cowUnei'wTa
2-ps
stetiuny tphase
hwmotgraphy
c howutara phy
m)
-Staionmy Pnae = Solicl adsbont (siica sel or e)uini
phase USucll an og <olWon
2x Column chsmologphy i tiin layo chomote py (7<)
u) P a t i o n chromotoaphy
mole pha\e
Hence
ex. Papo chromoto qraphy
seperati,
priiple Diffeyontial adsov ptiun
adsovod an givon ad Soybent
f Different compound
abbod more play adsoens
ike
a) coonnn hiono tu graphy.
can larye Scal Sepa t i o n t co mpondy
Seperetm o Com puundi ccUrs Column ot uds hont
glss e
ue to placod
The moVe
olun, a obile Is alowad t0
adsoybent
calumn. 9ramic
dissolv ed n ne sulvont
wi
CoMpQUDS
pOPIfICATIOM
-lo Se peraba
ImpuYitie
SUdinable orqanic cowpoun
Cam phor.
bexachloro naphnqloneanacene,antraquinone,
onzoie aid,
eane
( ) apvsTALLIGATl0
-A most general me oa t
- |4
poify ooid orqanic com pound
USine a Solvont "1n hich toe eom pound 1s paiincghy
Solble at tempoature but a pvecia bly dubte t bcn
4empat r
e eampund grcadualy Cwystallsa t
from leavina b bind all ne
u) DisWLLATGnN
Ponf Loklqle organic compound TUquid)
typen
(a) Gmple dsillation:
Se perale uolalile CompoUnd tom nen \lobile imponttey
Sepeate too misible iqvid i t consdeae ufîerencQ
bolina pont (>2 6c)
" cslo o fom C6c) aniune Cbp-lB4C)
dueyl ehcy (35O u Toluene ( l l ° )
mDtue
teront te mpe qtey.
e t o uaic la po|se a t
Pindple
bolin poln Wauid \apouI Se
fist Te a r s are
coleetd
Ondensec
(b) fractonal Disilla|in
wusscible Liaui
To Gepeyate two
elone
pe to leum\nity
hati ons
Pansed
e Vapuu
Sas temparcat
clunn
, hng eslumn ol multple su face
(colmn)
cc ) acuv m
Diillatn (Drtllatlon ond a preUre ):
oY
made u bol at loe empaetre boy
Phnciple; A s exkmal pre ssue derrere
(d) Steam istilla thon:
-to arl stea Wol atile and ond 'imide
wit a ey
, o no- nito phenl Sal caldehucle 6rho hydoy aetprn
Tbe iaui naving o-\S
callec steam Molaile Liq
at 100C
S4eam vog he bated '1mpure Sam ple ofstea
Volatile poundl slapouy of stean Valatk
(iqvid wlll
pnncsle A always bals a
The nGrnal
a
components
Condiion wwales volatl Pat.
loecawSe
(i) PIFERENWAL TXTRACI0N
- Done by aqveo medOm
rmxtu
) tc solent wwst be
IwwrsCble wnate.
nan
, " chloro oricnloromnehane
Ae more cdenso an atei
Jater mcible soluont al cannol be sed,, foy Aferontal ox traos
e hanol Calcohols), acetoe, diehel etes
(V) CHPOMAT0aPAPHY
- A Veny gonerGl me hod
Mus moden me thsd
miIte
phase is alowed to moe slouly t n he
Staoney nae 3Yaduly 3t
Seperaleel ver e statñonv hoye Cau Sa
cowUnei'wTa
2-ps
stetiuny tphase
hwmotgraphy
c howutara phy
m)
-Staionmy Pnae = Solicl adsbont (siica sel or e)uini
phase USucll an og <olWon
2x Column chsmologphy i tiin layo chomote py (7<)
u) P a t i o n chromotoaphy
mole pha\e
Hence
ex. Papo chromoto qraphy
seperati,
priiple Diffeyontial adsov ptiun
adsovod an givon ad Soybent
f Different compound
abbod more play adsoens
ike
a) coonnn hiono tu graphy.
can larye Scal Sepa t i o n t co mpondy
Seperetm o Com puundi ccUrs Column ot uds hont
glss e
ue to placod
The moVe
olun, a obile Is alowad t0
adsoybent
calumn. 9ramic
dissolv ed n ne sulvont
wi
