ASCP CG Exam
(Molecular CG Testing)
Questions and Complete
Solutions Graded A+
Denning [Date] [Course title]
, Which counterstain dye should be used to counterstain cells with Spectrum Green/Spectrum Red dual
color signals? - Answer: DAPI.
DAPI is a _______ counterstain. - Answer: Blue.
The process of FISH is used to: - Answer: Visualize a specific sequence of DNA with a fluorochrome.
Which is critical to the success of a FISH procedure? - Answer: Temperature of the denature solution.
Formamide is used in the FISH procedure for which of the following purposes? - Answer: To lower the
DNA melting point.
What is the purpose of post hybridization wash? - Answer: Remove unbound probe from the slide.
What is true about slide prep for FISH? - Answer: Slides should be kept at room temp conditions.
When analyzing a sample stained with a FISH probe, the technologist notices a high amount of
nonspecific cross hybridization. Which is most likely the cause? - Answer: The stringency was way too
low.
When using the Applied Imaging system to capture the FISH image, which program should be chosen to
use? - Answer: Probe.
Which of the following statements is true about directly labeled probes? - Answer: Exposure to light
should be avoided.
All of the following are advantages of array-CGH except? - Answer: The use of metaphase
chromosomes.
Which of the following regarding test resolution is correct? - Answer: BAC array has lower resolution
than oligo array.
(Molecular CG Testing)
Questions and Complete
Solutions Graded A+
Denning [Date] [Course title]
, Which counterstain dye should be used to counterstain cells with Spectrum Green/Spectrum Red dual
color signals? - Answer: DAPI.
DAPI is a _______ counterstain. - Answer: Blue.
The process of FISH is used to: - Answer: Visualize a specific sequence of DNA with a fluorochrome.
Which is critical to the success of a FISH procedure? - Answer: Temperature of the denature solution.
Formamide is used in the FISH procedure for which of the following purposes? - Answer: To lower the
DNA melting point.
What is the purpose of post hybridization wash? - Answer: Remove unbound probe from the slide.
What is true about slide prep for FISH? - Answer: Slides should be kept at room temp conditions.
When analyzing a sample stained with a FISH probe, the technologist notices a high amount of
nonspecific cross hybridization. Which is most likely the cause? - Answer: The stringency was way too
low.
When using the Applied Imaging system to capture the FISH image, which program should be chosen to
use? - Answer: Probe.
Which of the following statements is true about directly labeled probes? - Answer: Exposure to light
should be avoided.
All of the following are advantages of array-CGH except? - Answer: The use of metaphase
chromosomes.
Which of the following regarding test resolution is correct? - Answer: BAC array has lower resolution
than oligo array.
