ASCP MB Exam
Practice Questions
and Complete
Solutions Graded
A+
Denning [Date] [Course title]
,Mutation in UGT1A1 affects metabolism of what drug?
Irontecan
Phenobarbital
Indinavir
Sorafenib - Answer: Irontecan
Its discovery shed light on why there is simultaneous, though not continuous, synthesis of DNA on both
leading and lagging strands of DNA:
Klenow fragment of DNA polymerase
Okazaki fragments
Sanger fragments
RNA fragments - Answer: Okazaki fragments
What gene is measured following treatment with Warfarin?
FLT3
VKORC1
MAPK
P53 - Answer: VKORC1
What stain binds to minor groove of DNA Duplex?
SYBR1
EtBR
Propidium iodide
Methyl green - Answer: SYBR1
A parent has an autosomal dominant disorder. What percent chance does this parent have to pass down
this affected gene to his/her child?
0%
25%
, 50%
75%
100% - Answer: 50%
The bond between a phosphate group and the ribose sugar group in RNA is called which of the
following?
Phosphodiester linkage
Glycosidic bond
Peptide bond
Amide bond - Answer: Glycosidic bond
Branched DNA amplification (bDNA) is best classified as:
Signal amplification
Target amplification
Probe amplification
None of the above - Answer: Signal amplification
In which of the following methods is the probe digested by the polymerase (Taq) during primer
extension?
Taqman
FRET probes
Molecular beacons
Scorpion probes - Answer: Taqman
A patient with impaired judgment, personality changes, signs of abnormal body movements and
depression comes to the physician's office for a follow-up visit. The physician suspects a single-gene
disorder may be the cause of those clinical manifestations. A blood specimen was then sent to your
clinical laboratory for mutation screening in the Huntingtin gene. Testing with standard PCR indicates
that patient has Huntington Disease, HD. Which of the following would be consistent with this
diagnosis?
Practice Questions
and Complete
Solutions Graded
A+
Denning [Date] [Course title]
,Mutation in UGT1A1 affects metabolism of what drug?
Irontecan
Phenobarbital
Indinavir
Sorafenib - Answer: Irontecan
Its discovery shed light on why there is simultaneous, though not continuous, synthesis of DNA on both
leading and lagging strands of DNA:
Klenow fragment of DNA polymerase
Okazaki fragments
Sanger fragments
RNA fragments - Answer: Okazaki fragments
What gene is measured following treatment with Warfarin?
FLT3
VKORC1
MAPK
P53 - Answer: VKORC1
What stain binds to minor groove of DNA Duplex?
SYBR1
EtBR
Propidium iodide
Methyl green - Answer: SYBR1
A parent has an autosomal dominant disorder. What percent chance does this parent have to pass down
this affected gene to his/her child?
0%
25%
, 50%
75%
100% - Answer: 50%
The bond between a phosphate group and the ribose sugar group in RNA is called which of the
following?
Phosphodiester linkage
Glycosidic bond
Peptide bond
Amide bond - Answer: Glycosidic bond
Branched DNA amplification (bDNA) is best classified as:
Signal amplification
Target amplification
Probe amplification
None of the above - Answer: Signal amplification
In which of the following methods is the probe digested by the polymerase (Taq) during primer
extension?
Taqman
FRET probes
Molecular beacons
Scorpion probes - Answer: Taqman
A patient with impaired judgment, personality changes, signs of abnormal body movements and
depression comes to the physician's office for a follow-up visit. The physician suspects a single-gene
disorder may be the cause of those clinical manifestations. A blood specimen was then sent to your
clinical laboratory for mutation screening in the Huntingtin gene. Testing with standard PCR indicates
that patient has Huntington Disease, HD. Which of the following would be consistent with this
diagnosis?
