Gel Electrophoresis Online Simulation – Questions
With Accurate Solutions
What is electrophoresis used for? Right Ans - Pushing DNA strands through
the gel filter (Moving DNA)
What is the material of the gel similar to? Right Ans - A sponge made of
Jello
Describe how the DNA fragments migrate through the gel matrix. Right Ans
- DNA strands of the same length will move at the same speed and group up
together, they move through holes in the sponge.
What moves farther - shorter or longer fragments? Why? Right Ans -
Shorter because they move quicker
If two lanes had bands/fragments at the same location could you assume that
the DNA at these locations was the same length? Why or why not? Right
Ans - Yes because those bands moved the same distance meaning the the
same length because different length fragments move different distances.
What ingredients/materials are necessary to perform an electrophoresis
experiment? Right Ans - Powdered agarose, buffer, a flask, a microwave,
the gel mold and the gel comb.
Why do you need to use buffer? Right Ans - A buffer is a salt water solution
that will let electrical charges flow through the gel.
Why do you need a microwave? Could you use something else? What?
Right Ans - To heat and melt the agarose and buffer together. yes, You could
also use a hot plate and hot plate stirrer.
What is the tape for on the gel casting tray? Right Ans - To hold the melted
agarose
What is the comb for? Right Ans - To create wells
With Accurate Solutions
What is electrophoresis used for? Right Ans - Pushing DNA strands through
the gel filter (Moving DNA)
What is the material of the gel similar to? Right Ans - A sponge made of
Jello
Describe how the DNA fragments migrate through the gel matrix. Right Ans
- DNA strands of the same length will move at the same speed and group up
together, they move through holes in the sponge.
What moves farther - shorter or longer fragments? Why? Right Ans -
Shorter because they move quicker
If two lanes had bands/fragments at the same location could you assume that
the DNA at these locations was the same length? Why or why not? Right
Ans - Yes because those bands moved the same distance meaning the the
same length because different length fragments move different distances.
What ingredients/materials are necessary to perform an electrophoresis
experiment? Right Ans - Powdered agarose, buffer, a flask, a microwave,
the gel mold and the gel comb.
Why do you need to use buffer? Right Ans - A buffer is a salt water solution
that will let electrical charges flow through the gel.
Why do you need a microwave? Could you use something else? What?
Right Ans - To heat and melt the agarose and buffer together. yes, You could
also use a hot plate and hot plate stirrer.
What is the tape for on the gel casting tray? Right Ans - To hold the melted
agarose
What is the comb for? Right Ans - To create wells
