DAT LAB STUDY QUESTIONS AND ANSWERS WITH SOLUTIONS 2024

DAT LAB STUDY QUESTIONS AND
ANSWERS WITH SOLUTIONS 2024
what is the purpose of performing the DAT procedure? - ANSWER used to determine if red cells have
been coated in vivo with immunoglobulin, complement or both.



what does a positive DATE indicate? - ANSWER indicates that red cells are coated with protein in-vivo



what 3 reagents may be used in the DAT procedure? - ANSWER polyspecific IgG, C3d



monospecific IgG or C3d



what is the sample of choice for the DAT? Why? - ANSWER whole blood collected in an EDTA tube
because it prevents in-vitro fixation of complement.



the DAT is used to assist in hemolysis associated with what 4 clinical conditions: - ANSWER HDFN

autoimmune hemolytic anemia

red cell sensitization

transfusion rxns



what 3 items in the pt history are crucial in determining the clinical significance of a positive DAT? -
ANSWER pts diagnosis

transfusion and pregnancy hx

recent/current medications



state the principle of the DAT procedure - ANSWER the detection of abs that attached to red cells in-vivo



what ab class is involved in HDFN? - ANSWER IgG



what is the source of the specimen used for the DAT on newborns? - ANSWER cord blood sample