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Exam (elaborations)

BLOOD BANK-DAT QUESTIONS AND ANSWERS WITH SOLUTIONS 2024

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BLOOD BANK-DAT QUESTIONS AND ANSWERS WITH SOLUTIONS 2024

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BLOOD BANK-DAT QUESTIONS AND
ANSWERS WITH SOLUTIONS 2024
Direct Antiglobulin Test (DAT) - ANSWER -Detects IgG or complement bound to RBCs in vivo




-In the procedure, AHG reagent is added after the RBCs have been washed



-Agglutination demonstrates whether IgG or complement was attached to the RBCs



DAT false positive - ANSWER -Overcentrifugation

-Contaminated reagents

-Insufficient washing of RBCs

-Test tubes allowed to stand after centrifugation

-RBCs left in suspension for extended time before testing

-Clotted sample

-IV immune globulin

-Spontaneous agglutination (IgM)



DAT false negative - ANSWER -Improper cell washing (free antibody/complement remains and reacts
with AHG; no Check Cells react with antibodies)

-Delay in adding AHG after washing step (antibody elutes off and attaches to AHG)

-Inactive, or forgotten, AHG

-Improper specimen agitation before interpretation
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