(MB) ASCP STUDY EXAM QUESTIONS
AND ANSWERS WITH SOLUTIONS 2024
What is metagenome? - ANSWER Sampling the genome sequence of a community of organisms.
Polymorphism involved for Irinotecan? - ANSWER UGT1A1
NGS related to deletions and insertions, coverage, depth? - ANSWER -Paired-end reads for detection of
indels
-Coverage is how much of your target has been read
-Depth is how many times a unique position has been covered
Polymerase for lagging strand? - ANSWER Euk - DNA Pol Alpha
Pro - DNA Pol I
Polymerase for leading strand? - ANSWER Euk - DNA Pol Delta and DNA Pol Epsilon
Pro - DNA Pol III
Polymerase used in LAMP? - ANSWER -Loop-mediated Isothermal Amplification utilizes Bst Polymerase
-Has helicase activity and activity at annealing/elongating temps
Highest resistance gene in enterococcus? - ANSWER vanA has the highest resistance in enterococcus
Resistance in mecA gene in MRSA codes for? - ANSWER mecA responsible for antibiotic resistance in S.
aureus (MRSA), and codes for PBP2a, which has low affinity for beta-lactam antibiotics like penicillin.
Important in gene expression, defense in eukaryotes? - ANSWER Person guessed mRNA - gene-
expression and codes for proteins that bind to DNA to promote/inhibit expression.
Donor/Recipient - what's informative loci? - ANSWER No matching alleles is informative, you can tell D
from R; non-informative would be both D&R matching.
, Bond between DNA and histones? - ANSWER Ionic - DNA is negative and histones are positive
Amino Acids that make up histones? - ANSWER Arginine and Lysine - positively charged amino acids
Multiplex modifications for low yield? - ANSWER Person guessed KCl.
Increasing Mg2+ could increase yield.
Decreasing stringency increases yield, but increases chances of mispriming
PCR troubleshooting - ANSWER Increase Temp --> Increase Stringency
Increase Probe Length --> Increase Stringency
Increase Probe Complexity --> Increase Stringency
Lower Salt Conc. --> Increase Stringency
Remove Mg2+ --> Increase Stringency
Less Time --> Increase Stringency
Add Formamide --> Lowers Stringency
Add Bovine Serum --> Lowers Stringency
Increase Probe Conc. --> Lowers Stringency
How does an amplification control help? - ANSWER Differentiates a true negative from a false negative
Amplification controls have a target that should always amplify, even if your sample does not amplify
Best molecular assay for Cystic Fibrosis? - ANSWER CF is caused by a 3b deletion: delF508
RFLP, PCR and Southern Blot are used.
What to use FISH for: - ANSWER PWS/AS can be detected with FISH
Gaucher - Sequencing or enzyme activity screening
FXS - RFLP, PCR, SSCP
TS - Sequencing or enzyme activity screening
AND ANSWERS WITH SOLUTIONS 2024
What is metagenome? - ANSWER Sampling the genome sequence of a community of organisms.
Polymorphism involved for Irinotecan? - ANSWER UGT1A1
NGS related to deletions and insertions, coverage, depth? - ANSWER -Paired-end reads for detection of
indels
-Coverage is how much of your target has been read
-Depth is how many times a unique position has been covered
Polymerase for lagging strand? - ANSWER Euk - DNA Pol Alpha
Pro - DNA Pol I
Polymerase for leading strand? - ANSWER Euk - DNA Pol Delta and DNA Pol Epsilon
Pro - DNA Pol III
Polymerase used in LAMP? - ANSWER -Loop-mediated Isothermal Amplification utilizes Bst Polymerase
-Has helicase activity and activity at annealing/elongating temps
Highest resistance gene in enterococcus? - ANSWER vanA has the highest resistance in enterococcus
Resistance in mecA gene in MRSA codes for? - ANSWER mecA responsible for antibiotic resistance in S.
aureus (MRSA), and codes for PBP2a, which has low affinity for beta-lactam antibiotics like penicillin.
Important in gene expression, defense in eukaryotes? - ANSWER Person guessed mRNA - gene-
expression and codes for proteins that bind to DNA to promote/inhibit expression.
Donor/Recipient - what's informative loci? - ANSWER No matching alleles is informative, you can tell D
from R; non-informative would be both D&R matching.
, Bond between DNA and histones? - ANSWER Ionic - DNA is negative and histones are positive
Amino Acids that make up histones? - ANSWER Arginine and Lysine - positively charged amino acids
Multiplex modifications for low yield? - ANSWER Person guessed KCl.
Increasing Mg2+ could increase yield.
Decreasing stringency increases yield, but increases chances of mispriming
PCR troubleshooting - ANSWER Increase Temp --> Increase Stringency
Increase Probe Length --> Increase Stringency
Increase Probe Complexity --> Increase Stringency
Lower Salt Conc. --> Increase Stringency
Remove Mg2+ --> Increase Stringency
Less Time --> Increase Stringency
Add Formamide --> Lowers Stringency
Add Bovine Serum --> Lowers Stringency
Increase Probe Conc. --> Lowers Stringency
How does an amplification control help? - ANSWER Differentiates a true negative from a false negative
Amplification controls have a target that should always amplify, even if your sample does not amplify
Best molecular assay for Cystic Fibrosis? - ANSWER CF is caused by a 3b deletion: delF508
RFLP, PCR and Southern Blot are used.
What to use FISH for: - ANSWER PWS/AS can be detected with FISH
Gaucher - Sequencing or enzyme activity screening
FXS - RFLP, PCR, SSCP
TS - Sequencing or enzyme activity screening
