ASCP BLOOD BANK Questions with Correct
Answers
serum contains this ab more than others Correct Answer-IgG
this ab is majorly found in body secretions Correct Answer-IgA
the ___ portion of an antibody is responsible for placental transfer of
IgG, is responsible for complement fixation and causes problems for the
baby Correct Answer-Fc portion of IgG
these antibodies activate the classical complement pathway Correct
Answer-IgG, IgM
___ ab reacts at 37C (body temp) while _____ ab reacts at room temp
Correct Answer-IgG (body), IgM (room)
IgM Correct Answer--can activate classical pathway of complement
system
-only one molecule is necessary to activate complement
-results in hemolysis of RBCs and intravascular destruction (IV
hemolysis)
-ABO abs are typically IgM and can cause rapid hemolysis if an
incompatible unit is transfused
-expected in plasma, important in reverse typing
-these abs are IgM: Lewis, Ii, P, MNS
,-react best at ambient/room temp (22-24C)
-can interfere with detection of clinically significant IgG abs by masking
reactivity
-exist in moth monomeric and polymeric forms as pentamers containing
a J chain. These large molecules cause visible agglutination reactions of
ag-positive RBCs in saline
-pentameric form can be dissociated through cleavage of its covalent
bonds at the J chain using sulfhydral reducing agents
-IgM is removed by treatment with 2-ME and DTT
IgA Correct Answer--exists in both monomer and polymer form as
dimers or trimers composed of identical monomers
-about 30% of anti-A and anti-B antibodies are IgA class
-present in large amounts in body secretions
IgE Correct Answer--found only in monomeric form in trace
concentrations in serum. Important in allergic reactions
-Fc portion of IgE molecule attaches to basophils and mast cells, which
facilitates histamine release
-not known for hemolytic transfusion reactions
intravascular hemolysis occurs with ___ antibodies Correct Answer--
alloantibodies if complement is triggered
alloantibodies Correct Answer--antibodies produced after exposure to
genetically different, non-self of the same species
,Paroxysmal cold hemoglobinuria occurs with ____ Correct Answer-
autoantibodies- autoimmune disorder under cold temperatures.
extravascular hemolysis Correct Answer--occurs in the liver/spleen
-occurs as a result of abs coating RBCs (either self or non-self)
-removed by mononuclar phagocyte system
-split products of complement activation can stimulate the
reticuloendothelial system (RES)
human leukocyte antigens (HLAs) are _____ Correct Answer-
glycoproteins
most clinically significant blood group antigens and immunogenicity
(chance of body making antibody against it) Correct Answer--D (Rh) -
50%
-K (Kell) - 5%
-c (Rh)-2.05
-E (Rh) -decreased from here
-k (kell)
-e (Rh)
-Fya (Duffy)
-C (Rh)
-Jka (Kidd)
-S (MNSs)
, -Jkb (Kidd)
-s (MNSs)
antibodies against this blood group antigen tends to fall below detection
limit of titer Correct Answer-Kidd (Jka)
** ask if patient has ever received a transfusion and from where!!
factors that influence Ag-Ab reactions Correct Answer--centrifugation
-Ag-Ab ratio
-pH
-temperature
IgM antibodies that typically react in the immediate spin phase Correct
Answer-A, B, H, I, P1, M, N, Le^a, Le^b
IgG antibodies that react after the antiglobulin phase (37C) Correct
Answer-D, C, E, c, e, K, Fy, Jk, S, s, Le^1, Le^b
Factors that influence agglutination reactions Correct Answer--22%
albumin
-LISS
-PeG
-proteolytic enzymes
-chemical reduction (DTT, B-2-ME)
Answers
serum contains this ab more than others Correct Answer-IgG
this ab is majorly found in body secretions Correct Answer-IgA
the ___ portion of an antibody is responsible for placental transfer of
IgG, is responsible for complement fixation and causes problems for the
baby Correct Answer-Fc portion of IgG
these antibodies activate the classical complement pathway Correct
Answer-IgG, IgM
___ ab reacts at 37C (body temp) while _____ ab reacts at room temp
Correct Answer-IgG (body), IgM (room)
IgM Correct Answer--can activate classical pathway of complement
system
-only one molecule is necessary to activate complement
-results in hemolysis of RBCs and intravascular destruction (IV
hemolysis)
-ABO abs are typically IgM and can cause rapid hemolysis if an
incompatible unit is transfused
-expected in plasma, important in reverse typing
-these abs are IgM: Lewis, Ii, P, MNS
,-react best at ambient/room temp (22-24C)
-can interfere with detection of clinically significant IgG abs by masking
reactivity
-exist in moth monomeric and polymeric forms as pentamers containing
a J chain. These large molecules cause visible agglutination reactions of
ag-positive RBCs in saline
-pentameric form can be dissociated through cleavage of its covalent
bonds at the J chain using sulfhydral reducing agents
-IgM is removed by treatment with 2-ME and DTT
IgA Correct Answer--exists in both monomer and polymer form as
dimers or trimers composed of identical monomers
-about 30% of anti-A and anti-B antibodies are IgA class
-present in large amounts in body secretions
IgE Correct Answer--found only in monomeric form in trace
concentrations in serum. Important in allergic reactions
-Fc portion of IgE molecule attaches to basophils and mast cells, which
facilitates histamine release
-not known for hemolytic transfusion reactions
intravascular hemolysis occurs with ___ antibodies Correct Answer--
alloantibodies if complement is triggered
alloantibodies Correct Answer--antibodies produced after exposure to
genetically different, non-self of the same species
,Paroxysmal cold hemoglobinuria occurs with ____ Correct Answer-
autoantibodies- autoimmune disorder under cold temperatures.
extravascular hemolysis Correct Answer--occurs in the liver/spleen
-occurs as a result of abs coating RBCs (either self or non-self)
-removed by mononuclar phagocyte system
-split products of complement activation can stimulate the
reticuloendothelial system (RES)
human leukocyte antigens (HLAs) are _____ Correct Answer-
glycoproteins
most clinically significant blood group antigens and immunogenicity
(chance of body making antibody against it) Correct Answer--D (Rh) -
50%
-K (Kell) - 5%
-c (Rh)-2.05
-E (Rh) -decreased from here
-k (kell)
-e (Rh)
-Fya (Duffy)
-C (Rh)
-Jka (Kidd)
-S (MNSs)
, -Jkb (Kidd)
-s (MNSs)
antibodies against this blood group antigen tends to fall below detection
limit of titer Correct Answer-Kidd (Jka)
** ask if patient has ever received a transfusion and from where!!
factors that influence Ag-Ab reactions Correct Answer--centrifugation
-Ag-Ab ratio
-pH
-temperature
IgM antibodies that typically react in the immediate spin phase Correct
Answer-A, B, H, I, P1, M, N, Le^a, Le^b
IgG antibodies that react after the antiglobulin phase (37C) Correct
Answer-D, C, E, c, e, K, Fy, Jk, S, s, Le^1, Le^b
Factors that influence agglutination reactions Correct Answer--22%
albumin
-LISS
-PeG
-proteolytic enzymes
-chemical reduction (DTT, B-2-ME)
