MICROBIOLOGY EXAM- SLCC LISA
DAVIS QUESTIONS AND ANSWERS
Which of the following is true of archaea that produce methane? - Answer-They live in
the most extreme environments
Archaea and Bacteria are most similar in terms of their _______? - Answer-Unicellular
structure
Which of these deeply branching bacteria is considered a polyextremophile? - Answer-
Deinococcus radiodurans`
The term "deeply branching" refers to which of the following? - Answer-The position in
the evolutionary tree of deeply branching bacteria
Which of the following bacterial species is classified as high G+C gram-positive? -
Answer-Corynebacterium diphtheriae
Which of the following refers to photosynthesis performed by bacteria with the use of
water as the donor of electrons? - Answer-Oxygenic
Which of the following bacteria are the most prevalent in the human gut? - Answer-
Bacteroides
Which of the folliwing is the organelle that spirochetes use to propel themselves? -
Answer-Axial filament
Haemophilus influenzar is a common cause of which of the following? - Answer-Upper
respiratory tract infections
Class Betaproteobacteria includes all but which of the following genera? - Answer-
Campylobacter
All Alphaproteobacteria are which of the following? - Answer-Oligotrophs
Which of the following describes proteobacteria in domain bacteria? - Answer-Phylum
Which of the following refers to the type of interaction between two prokaryotic
populations in which one population benefits and the other is not affected? - Answer-
Commensalism
The term microbiota refers to which of the following? - Answer-All microorganisms in a
certain region of the human body
, The term prokaryotes refers to which of the following? - Answer-Unicellular organisms
that have no nucleus
Archae - Answer-Found in extreme environments
Alphabacteria - Answer-Can grow in low nutrient environments
Betaproteobacteria - Answer-Requires lots of nutrients
Gammaproteobacteria - Answer-Largest and most diverse class of proteobacteria
Epsilonproteobacteria - Answer-Contains pathogens that cause ulcers and food
poisoning
green and purple sulfur bacteria - Answer-Photosynthetic, do not produce oxygen
Actinobacteria - Answer-high G+C gram positive bacteria
Gammaproteobacteria ex. - Answer-Haemophilus influenzae (Upper Resp. Tract infect.)
Nonproteobacteria ex - Answer-Borrelia burgdorferi
Betaproteobacteria ex - Answer-Bordetella pertusis
Firmicute ex - Answer-Clostridium
Rickettsia ex. - Answer-Alphaproteobacteria
Gram stain procedure - Answer-1. Primary stain (Crystal violet- a basic stain)
2. Mordant (Gram's iodine- I2 + KI)
(something that helps fix dye on or in a cell but does not itself contribute color)
3. decolorizing agent (Ethanol or acetone)
4. Counterstain (Safranin- a basic stain)
Gram stain results - Answer-Gram + = purple
Gram - = pink/red
acid fast stain - Answer-1. Primary stain (Fuchsin)
2.Acid-alcohol
3 Counterstained (Methylene blue)
Acid fast results - Answer-Acid Fast=Red
Non-acid-fast=Blue
endospore stain - Answer-1.Heat Fixed & stained (Malachite green)
2.Washed
DAVIS QUESTIONS AND ANSWERS
Which of the following is true of archaea that produce methane? - Answer-They live in
the most extreme environments
Archaea and Bacteria are most similar in terms of their _______? - Answer-Unicellular
structure
Which of these deeply branching bacteria is considered a polyextremophile? - Answer-
Deinococcus radiodurans`
The term "deeply branching" refers to which of the following? - Answer-The position in
the evolutionary tree of deeply branching bacteria
Which of the following bacterial species is classified as high G+C gram-positive? -
Answer-Corynebacterium diphtheriae
Which of the following refers to photosynthesis performed by bacteria with the use of
water as the donor of electrons? - Answer-Oxygenic
Which of the following bacteria are the most prevalent in the human gut? - Answer-
Bacteroides
Which of the folliwing is the organelle that spirochetes use to propel themselves? -
Answer-Axial filament
Haemophilus influenzar is a common cause of which of the following? - Answer-Upper
respiratory tract infections
Class Betaproteobacteria includes all but which of the following genera? - Answer-
Campylobacter
All Alphaproteobacteria are which of the following? - Answer-Oligotrophs
Which of the following describes proteobacteria in domain bacteria? - Answer-Phylum
Which of the following refers to the type of interaction between two prokaryotic
populations in which one population benefits and the other is not affected? - Answer-
Commensalism
The term microbiota refers to which of the following? - Answer-All microorganisms in a
certain region of the human body
, The term prokaryotes refers to which of the following? - Answer-Unicellular organisms
that have no nucleus
Archae - Answer-Found in extreme environments
Alphabacteria - Answer-Can grow in low nutrient environments
Betaproteobacteria - Answer-Requires lots of nutrients
Gammaproteobacteria - Answer-Largest and most diverse class of proteobacteria
Epsilonproteobacteria - Answer-Contains pathogens that cause ulcers and food
poisoning
green and purple sulfur bacteria - Answer-Photosynthetic, do not produce oxygen
Actinobacteria - Answer-high G+C gram positive bacteria
Gammaproteobacteria ex. - Answer-Haemophilus influenzae (Upper Resp. Tract infect.)
Nonproteobacteria ex - Answer-Borrelia burgdorferi
Betaproteobacteria ex - Answer-Bordetella pertusis
Firmicute ex - Answer-Clostridium
Rickettsia ex. - Answer-Alphaproteobacteria
Gram stain procedure - Answer-1. Primary stain (Crystal violet- a basic stain)
2. Mordant (Gram's iodine- I2 + KI)
(something that helps fix dye on or in a cell but does not itself contribute color)
3. decolorizing agent (Ethanol or acetone)
4. Counterstain (Safranin- a basic stain)
Gram stain results - Answer-Gram + = purple
Gram - = pink/red
acid fast stain - Answer-1. Primary stain (Fuchsin)
2.Acid-alcohol
3 Counterstained (Methylene blue)
Acid fast results - Answer-Acid Fast=Red
Non-acid-fast=Blue
endospore stain - Answer-1.Heat Fixed & stained (Malachite green)
2.Washed
