ASU BIO 340-
Exam 3 Module 6
Questions and
Complete Answers
Graded A+
Denning [Date] [Course title]
, The PCR step during which the double-stranded template molecule separates into two strands is called
_____________.
a. denaturation
b. annealing
c. extension - Correct Ans: a
In agarose gel electrophoresis, DNA will be attracted to the negative electrode.
a. True
b. False - Correct Ans: b
Which of the following are true about DNA and gel electrophoresis?
a. DNA is negatively charged
b. Small bands travel more quickly than large bands
c. The size of a DNA fragment is measured in base pairs
d. All of the above - Correct Ans: d
Regarding Sanger's sequencing method, why does the incorporation of a ddNTP in the growing
complementary strand terminates the process of DNA replication?
a. Because the ddNTP cannot form hydrogen bonds with the complementary base in the template
strand due to the missing hydroxyl group (-OH).
b. Because the ddNTP cannot form a peptide bond with the complementary base in the template strand
due to the missing hydroxyl group (-OH).
c. Because the ddNTP cannot form a phosphodiester bond with the sugar from the complementary base
in the template strand due to the missing hydroxyl group (-OH).
d. Because the ddNTP cannot form a phosphodiester bond with the sugar from a newly recruited dNTP
due to the missing hydroxyl group (-OH). - Correct Ans: d
Exam 3 Module 6
Questions and
Complete Answers
Graded A+
Denning [Date] [Course title]
, The PCR step during which the double-stranded template molecule separates into two strands is called
_____________.
a. denaturation
b. annealing
c. extension - Correct Ans: a
In agarose gel electrophoresis, DNA will be attracted to the negative electrode.
a. True
b. False - Correct Ans: b
Which of the following are true about DNA and gel electrophoresis?
a. DNA is negatively charged
b. Small bands travel more quickly than large bands
c. The size of a DNA fragment is measured in base pairs
d. All of the above - Correct Ans: d
Regarding Sanger's sequencing method, why does the incorporation of a ddNTP in the growing
complementary strand terminates the process of DNA replication?
a. Because the ddNTP cannot form hydrogen bonds with the complementary base in the template
strand due to the missing hydroxyl group (-OH).
b. Because the ddNTP cannot form a peptide bond with the complementary base in the template strand
due to the missing hydroxyl group (-OH).
c. Because the ddNTP cannot form a phosphodiester bond with the sugar from the complementary base
in the template strand due to the missing hydroxyl group (-OH).
d. Because the ddNTP cannot form a phosphodiester bond with the sugar from a newly recruited dNTP
due to the missing hydroxyl group (-OH). - Correct Ans: d
