MIC 102 Q3 STUDY GUIDE WITH COMPLETE SOLUTION!!

MIC 102 Q3 STUDY GUIDE WITH COMPLETE
SOLUTION!!
Describe the general strucutre of DNA
Answers :5' to 3'
anti parallel
Nucleotides
Why would a bacteria have more A-T vs G-C in their DNA?


Answers :A-T=want that DNA to be opened up
G-C=don't want the DNA to be as open
From where to where does replication of DNA move?


Answers :Form oriC to terC
What is the role of DnaA in replication?


Answers :Open up the DNA; binds to origin
What is the role of helicase (DnaB) in replication?


Answers :Unwind the DNA
What is the role of DnaC in replication?


Answers :Loads helicase onto DNA; uses ATP
What is the role of gyrase in replication?


Answers :Relieves tension ahead of helicase + seperates chromosomes at end

, What is the role of SSB proteins in replication?


Answers :Stabilize single stranded DNA
What is the role of primase in replication?


Answers :Lays down RNA primers
What is the role of DNA poly 3 in replication?


Answers :catalyzes most of DNA synthesis during replication (exonuclease)
What is the role of DNA poly 1 in replication?


Answers :Takes primers off
What is the role of ligase in replication?


Answers :Connects the Okazaki fragments via phosphodiester bonds
What is the role of Tus in replication?


Answers :Acts at termination of replication
What is the role topoisomerase IV in replication?


Answers :Seperates daughter molecules in final stages
Why does replication start at oriC?


Answers :It is AT-rich so it is easier to open up
Describe the initiation part of replication