Polymerase Chain Reaction Study Guide Exam And Actual Answers.

What needs to be done to the sample of blood before PCR? - Answer DNA must be isolated from the cells. How did you collect quids in the lab? - Answer A pipette What do you need to do each time before using a pipette to collect liquid? - Answer Put on a new, sterile pipette tip. What is the function of primers in a PCR reaction? - Answer Bind specific sites on DNA Great that you throw away the used pipette tip, why is it important to change the pipette tip? - Answer to avoid cross contamination For which enzymes are nucleotides the substrate? - Answer DNA Polymerase What is the template of the PCR reaction? - Answer DNA What does DNA polymerase do? - Answer Synthesizes DNA AT this step in the DNA process, what happens to the DNA? - Answer It will be separated into two stands. How is the DNA separated into a single strand? - Answer The high temperature 95° separates them. What is the step in the PCR reaction that is now shown called? - Answer Annealing The area where the primers bind marks which part of the PCR product? - Answer Beginning