Written by students who passed Immediately available after payment Read online or as PDF Wrong document? Swap it for free 4.6 TrustPilot
logo-home
Exam (elaborations)

DNA Methylation Methods and Protocols Second Edition

Rating
-
Sold
-
Pages
388
Grade
A+
Uploaded on
25-07-2024
Written in
2023/2024

Contents Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiii PART I. INTRODUCTION 1. DNA Methylation: An Introduction to the Biology and the Disease-Associated Changes of a Promising Biomarker J¨org Tost. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 PART II. GLOBAL METHYLATION LEVELS 2. Quantification of Global DNA Methylation by Capillary Electrophoresis and Mass Spectrometry Mar´ıa Berdasco, Mario F. Fraga, and Manel Esteller . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 3. Methyl Group Acceptance Assay for the Determination of Global DNA Methylation Levels Kenneth P. Nephew, Curt Balch, and David G. Skalnik . . . . . . . . . . . . . . . . . . . . . . . . . 35 PART III. METHODS FOR WHOLE GENOME ANALYSIS OF DNA METHYLATION PATTERNS 4. Immunodetection Array Johannes Pr¨oll, Christian Wechselberger, Mathilde F¨odermayr, Otto Zach, and Dieter Lutz . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45 5. Methylated DNA Immunoprecipitation (MeDIP) Fabio Mohn, Michael Weber, Dirk Sch¨ubeler, and Tim-Christoph Roloff . . . . . . . . . . 55 6. The MIRA Method for DNA Methylation Analysis Tibor A. Rauch and Gerd P. Pfeifer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65 7. The HELP Assay Mayumi Oda and John M. Greally . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77 8. Differential Methylation Hybridization: Profiling DNA Methylation with a High-Density CpG Island Microarray Pearlly S. Yan, Dustin Potter, Daniel E. Deatherage, Shili Lin, and Tim H.-M. Huang . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 9. Analysis of DNA Methylation by Amplification of Intermethylated Sites (AIMS) Mireia Jord`a, Jairo Rodriguez, Jordi Frigola, and Miguel A. Peinado . . . . . . . . . . . 107 10. Methylation-Sensitive Representational Difference Analysis (MS-RDA) Toshikazu Ushijima and Satoshi Yamashita . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117

Show more Read less
Institution
Course

Content preview

Springer Protocols
Methods in Molecular Biology 507




DNA Methylation
Methods and Protocols
Second Edition


Edited by

Jörg Tost

,DNA Methylation

, M E T H O D S I N M O L E C U L A R B I O L O G Y TM
John M. Walker, SERIES EDITOR
507. DNA Methylation: Methods and Protocols, Second 468. Wnt Signaling, Volume 1: Pathway Methods and
Edition, edited by Jörg Tost 2009 Mammalian Models, edited by Elizabeth Vincan,
502. Bacteriophages: Methods and Protocols, Volume 2: 2008
Molecular and Applied Aspects, edited by Martha 467. Angiogenesis Protocols: Second Edition, edited by
R. J. Clokie and Andrew M. Kropinski 2009 Stewart Martin and Cliff Murray, 2008
501. Bacteriophages: Methods and Protocols, Volume 1: 466. Kidney Research: Experimental Protocols, edited by
Isolation, Characterization, and Interactions, edited Tim D. Hewitson and Gavin J. Becker, 2008
by Martha R. J. Clokie and Andrew M. Kropinski 465. Mycobacteria, Second Edition, edited by Tanya
2009 Parish and Amanda Claire Brown, 2008
496. DNA and RNA Profiling in Human Blood: Methods 464. The Nucleus, Volume 2: Physical Properties and
and Protocols, edited by Peter Bugert, 2009 Imaging Methods, edited by Ronald Hancock, 2008
493. Auditory and Vestibular Research: Methods and Pro- 463. The Nucleus, Volume 1: Nuclei and Subnuclear
tocols, edited by Bernd Sokolowski, 2009 Components, edited by Ronald Hancock, 2008
489. Dynamic Brain Imaging: Methods and Protocols, 462. Lipid Signaling Protocols, edited by Banafshe
edited by Fahmeed Hyder, 2009 Larijani, Rudiger Woscholski, and Colin A. Rosser,
485. HIV Protocols: Methods and Protocols, edited by 2008
Vinayaka R. Prasad and Ganjam V. Kalpana, 2009 461. Molecular Embryology: Methods and Protocols,
484. Functional Proteomics: Methods and Protocols, Second Edition, edited by Paul Sharpe and Ivor
edited by Julie D. Thompson, Christine Schaeffer- Mason, 2008
Reiss, and Marius Ueffing, 2008 460. Essential Concepts in Toxicogenomics, edited by
483. Recombinant Proteins From Plants: Methods and Donna L. Mendrick and William B. Mattes, 2008
Protocols, edited by Loı̈c Faye and Veronique 459. Prion Protein Protocols, edited by Andrew F. Hill,
Gomord, 2008 2008
482. Stem Cells in Regenerative Medicine: Methods and 458. Artificial Neural Networks: Methods and Applica-
Protocols, edited by Julie Audet and William L. tions, edited by David S. Livingstone, 2008
Stanford, 2008
457. Membrane Trafficking, edited by Ales Vancura,
481. Hepatocyte Transplantation: Methods and Protocols, 2008
edited by Anil Dhawan and Robin D. Hughes, 2008
456. Adipose Tissue Protocols, Second Edition, edited
480. Macromolecular Drug Delivery: Methods and Pro- by Kaiping Yang, 2008
tocols, edited by Mattias Belting, 2008
455. Osteoporosis, edited by Jennifer J. Westendorf, 2008
479. Plant Signal Transduction: Methods and Protocols,
edited by Thomas Pfannschmidt, 2008 454. SARS- and Other Coronaviruses: Laboratory Proto-
cols, edited by Dave Cavanagh, 2008
478. Transgenic Wheat, Barley and Oats: Production and
Characterization Protocols, edited by Huw D. Jones 453. Bioinformatics, Volume 2: Structure, Function, and
and Peter R. Shewry, 2008 Applications, edited by Jonathan M. Keith, 2008
477. Advanced Protocols in Oxidative Stress I, edited by 452. Bioinformatics, Volume 1: Data, Sequence Analysis,
Donald Armstrong, 2008 and Evolution, edited by Jonathan M. Keith, 2008
476. Redox-Mediated Signal Transduction: Methods and 451. Plant Virology Protocols: From Viral Sequence to
Protocols, edited by John T. Hancock, 2008 Protein Function, edited by Gary Foster, Elisabeth
Johansen, Yiguo Hong, and Peter Nagy, 2008
475. Cell Fusion: Overviews and Methods, edited by
Elizabeth H. Chen, 2008 450. Germline Stem Cells, edited by Steven X. Hou and
Shree Ram Singh, 2008
474. Nanostructure Design: Methods and Protocols,
edited by Ehud Gazit and Ruth Nussinov, 2008 449. Mesenchymal Stem Cells: Methods and Protocols,
edited by Darwin J. Prockop, Douglas G. Phinney,
473. Clinical Epidemiology: Practice and Methods, and Bruce A. Brunnell, 2008
edited by Patrick Parfrey and Brendon Barrett,
2008 448. Pharmacogenomics in Drug Discovery and Devel-
opment, edited by Qing Yan, 2008
472. Cancer Epidemiology, Volume 2: Modifiable Fac-
tors, edited by Mukesh Verma, 2008 447. Alcohol: Methods and Protocols, edited by
Laura E. Nagy, 2008
471. Cancer Epidemiology, Volume 1: Host Susceptibility
Factors, edited by Mukesh Verma, 2008 446. Post-translational Modifications of Proteins: Tools
for Functional Proteomics, Second Edition, edited by
470. Host-Pathogen Interactions: Methods and Protocols, Christoph Kannicht, 2008
edited by Steffen Rupp and Kai Sohn, 2008
445. Autophagosome and Phagosome, edited by Vojo
469. Wnt Signaling, Volume 2: Pathway Models, edited Deretic, 2008
by Elizabeth Vincan, 2008

, TM
METHODS IN MOLECULAR BIOLOGY




DNA Methylation
Methods and Protocols

Second Edition


Edited by

Jörg Tost, PhD
CEA - Institut de Génomique, Evry, France

Written for

Course

Document information

Uploaded on
July 25, 2024
Number of pages
388
Written in
2023/2024
Type
Exam (elaborations)
Contains
Questions & answers

Subjects

$28.49
Get access to the full document:

Wrong document? Swap it for free Within 14 days of purchase and before downloading, you can choose a different document. You can simply spend the amount again.
Written by students who passed
Immediately available after payment
Read online or as PDF

Get to know the seller

Seller avatar
Reputation scores are based on the amount of documents a seller has sold for a fee and the reviews they have received for those documents. There are three levels: Bronze, Silver and Gold. The better the reputation, the more your can rely on the quality of the sellers work.
THEEXCELLENCELIBRARY Harvard University
Follow You need to be logged in order to follow users or courses
Sold
18
Member since
2 year
Number of followers
6
Documents
2641
Last sold
2 months ago
THE EXCELLENCE LIBRARY

The Excellence Library Where Academic Success Begins. Welcome to The Excellence Library — your trusted marketplace for past and upcoming exam papers with verified answers, spanning all academic fields. Whether you're a med student, a future lawyer, a high schooler prepping for finals, or a researcher looking for model dissertations — we've got you covered. What We Offer Accurate & Complete Exam Papers From Medicine, Nursing, Law (Bar Exams), High School subjects, and more. Model Dissertations & Novels Top-tier academic references and full-text materials to guide your writing and study. Affordable & Fair Pricing Quality resources at a price that respects students' budgets. Why Choose Us? Thoroughly Reviewed Answers – Every paper includes clear, correct solutions. Massive Library – Thousands of documents, constantly updated. Academic Excellence, Delivered – We help you prepare smarter, not harder. Fast Delivery – Get what you need, when you need it. Our Goal To empower students and professionals by offering reliable, affordable academic materials — helping you succeed one paper at a time.

Read more Read less
2.5

2 reviews

5
0
4
0
3
1
2
1
1
0

Trending documents

Recently viewed by you

Why students choose Stuvia

Created by fellow students, verified by reviews

Quality you can trust: written by students who passed their tests and reviewed by others who've used these notes.

Didn't get what you expected? Choose another document

No worries! You can instantly pick a different document that better fits what you're looking for.

Pay as you like, start learning right away

No subscription, no commitments. Pay the way you're used to via credit card and download your PDF document instantly.

Student with book image

“Bought, downloaded, and aced it. It really can be that simple.”

Alisha Student

Frequently asked questions