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BIOE 206 Exam 1 Questions and Answers 100% Correct

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BIOE 206 Exam 1 Questions and Answers 100% CorrectBIOE 206 Exam 1 Questions and Answers 100% CorrectBIOE 206 Exam 1 Questions and Answers 100% CorrectBIOE 206 Exam 1 Questions and Answers 100% CorrectBIOE 206 Exam 1 Questions and Answers 100% Correct Plasmid DNA purification steps - ANSWER-1. grow the bacteria with the plasmid 2. centrifuge 3. remove supernatant 4. resuspend pellet in lysis buffer (detergents and high salt) 5. neutralize the lysis buffer 6. spin down to remove debris and genomic DNA 7. put the suspension on a spin column 8. plasmid DNA will adhere to spin column and everything else will be washed away with high salt buffers 9. add low salt buffer to remove plasmid DNA from spin column RNA purification steps - ANSWER-Same steps as DNA purification, but also need to heat to disrupt any secondary structures. Since the mRNA is the only type that has a polyA tail, the beads can have a "polyT tail"

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  • bioe 206 exam 1 questions and answers stuvia
  • plasmid dna purification steps answer 1 grow
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