Biochem Engineering: Protein Expression in Eukaryotes Exam Questions With Verified Answers.

Biochem Engineering: Protein Expression in Eukaryotes Exam Questions With Verified Answers. Why are eukaryotic cells used - answerglycosylation and degraded/misfolded protein in bacterial system glycosylation - answersequence of carb chain incorporated on protein factors when deciding on a system - answer1. complexity of protein 2. level of glycosylation 3. promoter system availability three host cell systems used for expression - answeryeast, insect cells, mammalian cells yeast advantages - answer1. easy to grow and safe 2. good genetics 3. variety of promoters yeast disadvantage - answerlimited availability to glycosylate yeast promoters - answerGa11 and Ga110 three yeast vectors - answer1. YAC 2. 2 um plasmids 3. integrative plasmid yeast artificial chromosome (YAC) - answer1. key eleme