Biochem Engineering: Recombinant DNA Tech Exam Questions With Verified Answers.

Biochem Engineering: Recombinant DNA Tech Exam Questions With Verified Answers. Ways to cut DNA - answer1. chemical cleavage (random) 2. shearing (random) 3. restriction enzymes restriction endonucleases - answercut both strands of DNA duplex at a specific place restriction mapping - answerfor determining the locations of restriction endonuclease sites within a segment of DNA sum of fragment sizes equals... - answersize of unfragmented DNA molecule partial digestion - answerDNA incubated with a restriction enzyme for a short time so only fraction of cleavage site are cut DNA ligase - answeran enzyme that eventually joins the sugar-phosphate backbones of the Okazaki fragments DNA exonuclease - answerchew back sticky ends to make them blunt cloning vectors - answersmall piece of DNA used to introduce foreign gene into host cell; used to get multiple copies of foreign genes plasmid size - answer10 kbp expression vector - answerplasmid that introduces gene of interest and aids in protein product expression artificial chromosomes - answerBAC (300), YAC (1000), HAC (20,000) plasmids contain... - answer1. origin of replication 2. selectable markers selectable markers - answergenes encode