Ace ASCP Questions and Answers Rated A

Which of the following condition is the most common cause of increase anion gap? A.Metabolic alkalosis B. Metabolic acidosis Respiratory acidosis Respiratory alkalosis B. Metabolic acidosis High anion gap metabolic acidosis is caused generally by the body producing too much acid or not producing enough bicarbonate. This is often due to an increase in lactic acid or ketoacids, or it may be a sign of kidney failure. More rarely, high anion gap metabolic acidosis may be caused by ingesting methanol or overdosing on aspirin. Which of the following enzymes has the longest elevation after Myocardial Infarction (MI)? CK-MB Myoglobin Troponin AOTA C. Troponin Troponin is elevated up to 10-14 days after Myocardial Infarction. Lactic acid specimen: Chilled and separated from cells Heated Room temperature Request EDTA sample only A. Chilled and separated from cells Feedback Special Handling: No tourniquet. Place on ice immediately and deliver to lab or aliquot within 15 minutes. DO NOT FREEZE WHOLE BLOOD SPECIMEN. Specific gravity measured by refractometer: 1.035. Protein and glucose both trace in reagent strip: Presence X-ray medium Albuminuria Glucosuria Normal A. Presence X-Ray medium Feedback Xray medium/Radiographic dye can cause erroneous results in urine specific gravity measurement. A patient with walking pneumonia is being treated with penicillin. State one possible condition why the patient is not recovering: The organism produces beta lactamase The organism has no cell wall The organism has capsule The organism produces an enzyme that neutralizes the antibiotic B. The organism has no cell wall. Feedback Pneumonia caused by Mycoplasma pneumoniae is routinely treated with antibiotics, although the disease is usually self-limiting. The disease is treated with macrolide, tetracycline, or fluoroquinolone classes of antibiotics. All mycoplasmas lack a cell wall and, therefore, all are inherently resistant to beta-lactam antibiotics (e.g., penicillin). What is the coefficient of variation if the mean is 90 and the standard deviation is 5.48? 2.75 6.09 5.68 30 B. 6.09 Feedback: CV= SD/meanX100, (5.48/90)X100= 6.09. Blood smear below denotes Hairy Cell Leukemia (HCL), which of the following test is used to identify this type of leukemia? Image. 1 Myeloperoxidase Tartrate-resistant acid phosphatase Tartrate-resistant alkaline phosphatase Sudan Black B. Tartrate-resistant acid phosphatase Feedback: Hairy cells demonstrate strong positivity for tartrate-resistant acid phosphatase (TRAP) staining. The hairy cells are larger than normal and positive for CD19, CD20, CD22, CD11c, CD25, CD103, and FMC7. Use to differentiate Salmonella from Citrobacter: Lysine decarboxylase ONPG Indole Vogues Proskauerterm-9 A. Lysine decarboxylase Feedback: Salmonella (+); Citrobacter (-) Arrange the common Rh antigens according to immunogenicity (greatest to least): D>c>E>C>e D>C>E>c>e D>c>E>e>C C>D>c>E>e A. D>c>E>C>e Feedback Most immunogenic D > c > E > C > e least immunogenic. Most common error in Polymerase Chain Reaction (PCR) is NUCLEIC ACID CONTAMINATION. A True (Correct Answer) B False A. True What disease is associated with the possession of HLA-B27? Ankylosing spondylitis Hashimoto's Disease Goodpasture's syndrome SLE A. Ankylosing spondylitis Feedback: Ankylosing spondylitis belongs to a group of arthritis conditions that tend to cause chronic inflammation of the spine (spondyloarthropathies). The HLA-B27 gene can be detected in the blood of most patients with ankylosing spondylitis. Burr cells are seen in the peripheral blood smear, this is indicative of? (Fig. 2) Uremia (Kidney Disease) Anemia Leukemia Liver Disease A. Uremia (kidney Disease) Feedback: The above shown picture illustrates Burr cells, these cells are indicative of Uremia (kidney disease) Anti IgG (-); C3d (+): What should the MLS do? Elution IAT DAT Pre-warm D. Pre-warm Feedback Prewarm technique can be used to prevent cold-reactive alloantibodies or autoantibodies from reacting in the IAT phase. Specifically, prewarm technique prevents cold antibodies from binding complement at RT (as opposed to 37o C ) and subsequently being detected by anti-C3 in the IAT by polyspecific AHG serum. Maximum interval which a recipient sample maybe used for crossmatching if the patient has been recently transfused, has been pregnant? 2 days 6 days 3 days NOTA C. 3 days CIN (Cefsulodin, Irgasan, Novobiocin) Agar is a recommended culture medium for the recovery of: Rhodococcus equi Cardiobacterium hominis Brucella suis Yersinia enterocolitica D. Yersinia enterocolitica Feedback: CIN Agar is recommended for use in the selective and differential isolation of Yersinia and Aeromonas species from clinical specimens, environmental samples, and food sources. Yersinia enterocolitica will exhibit bull's eye appearance on the said agar. What is the formula for sensitivity