MMBIO 222 Quiz 1 Questions with Complete Solutions

Antonie van Leeuwenhoek Correct Answer--Invented the microscope -Dutch linen merchant, and microbiologist -Discovered the microbial world, coined the term animacules -Constructed over 250 small, powerful microscopes that could magnify up to 300 times -Known as the father of bacteriology and protozoology Resolving Power Correct Answer-Ability of a microscope to differentiate two closely spaced objects as being distinct from one another The light microscope can distinguish two points that are at least 0.2 um apart as distinct objects Calculation (N.A. = numerical aperture) R.P. = wavelength of light (λ)/2xN.A. = 500 nm/2x1.25 = 200 nm or 0.2 um Resolving power increases as magnification of the objective lens increases Use of a shorter wavelength of light increases resolving power Resolving power also depends on a second factor, the numerical aperture Numerical Aperture Correct Answer-is the ability of a lens in a light microscope to gather light ??= 1.25 for the oil immersion lens