BIOLOGY LAB 1107 KENNESAW STATE FINAL EXAM 2024 LATEST QUESTIONS & ANSWERS SOLVED 100% CORRECT!

Adaptability of Cells - specific proteins present and functioning at one given time are replaced or joined by new proteins. Lab 5 reaction Enzymology - PNPP + H2O -------------> PNP + Pi (colorless) (yellow) substrate Lab five Spectrometer set at what nm? - Make sure spectrophotometer is set to read absorbance at a wavelength of 410 nm. Change in OD = - 15 minute reading - zero minute reading Cellular respiration - The process that converts the energy in food molecules into the energy of ATP is known 1. Glycolysis: - sugar glucose are converted to three-carbon molecules called pyruvate. Intermediate Step: - Acetyl CoA is the starting point for the citric acid cycle. The Citric Acid Cycle - -acetyl CoA is broken down -eventually produce carbon dioxide and in the process generate reduced electron carriers (e.g. NADH and FADH2) The Electron Transport System (ETS): - oxidation-reduction reactionsWhere does the Citric Acid Cycle occur ? - The citric acid cycle occurs within mitochondria. Initial reaction catalyzed by succinate dehydrogenase - Succinate + FAD -----> fumarate + FADH2 Reaction 1: Lab 6 - Succinate + FAD ----------------------------> fumarate + FADH2 Reaction 2: Lab 6 - Rx. 2: FADH2 + DCIPox --------------------------> FAD + DCIPred (blue) (colorless) Prediction for Lab 6 cellular respiration: - If the citric acid cycle occurs within the mitochondria then the fraction with mitochondria will have the highest SA values. Calculation for Lab 6 cellular respiration OD: - OD at 15 minutes - OD at 0 minutes/ 15 minutes Calculate EU - Average OD/minute (from Step 3) x Total Dilution = micromoles catalyzed DCIP = ______ EU OD/micromoles DCIP (from Step 4) minute (from Step 2) SA in Enzyme Activity: - THE SA IS DEFINED AS THE NUMBER OF ENZYME UNITS (EU) PER MILLIGRAM OF TOTAL PROTEIN IN THE SAMPLE. mitosis - specifically refers to the partitioning of the chromosomes.Interphase G1 - the cell undergoes general growth Interphase S - the chromosomes are duplicated. Interphase G2 - the cell makes special structures