SBB Human Blood Groups Questions with Solutions, A+

How would Ulex europaeus react with O, A, A2, B, A2B, A1B RBCs? - O>A2>B>A2B>A1>A1B How could you distinguish serologically A1, A2 and A3 - Dolichos biflorus can will react with A1 and not A2. A3 yields mixed field reactions with anti-A and anti-A,B What is the correct order of type of RBCs to transfuse in an AB patient when AB RBCs are not available? Why? - A, B, O; the residual anti-B in A RBCs is less immunogenic than the residual anti-A in B RBCs What type of RBCs would you transfuse to a patient with anti-G - RBCs transfused must lack the D and C antigens Can you detect a weak D using Gel Technology? - No, because there is no washing phase Can an Rh positive father and Rh positive mother yield an Rh negative child - Yes, if both parents are heterozygous for the D gene. Inheritance is autosomal recessive What type of RBCs in regards to Rh phenotype would you transfuse in a patient with anti-f - RBCs should be negative for the c and e antigens How could you solve an ABO discrepancy where Anti-M was causing aberrant results in reverse grouping when the forward type is consistent with A blood group and reverse as O? - Treat A1 cells with ficin to denature the M antigen, repeat reverse grouping with ficin-treated A1 cells. Why do you see an anti-N like specificity in dialysis patients? - Formaldehyde induced antibody to 'N' due to formaldehyde used in dialysis procedures. A patient types as follows: anti-A=4+, Anti-B=1+, A1 Cells=2+, B cells=4+. The ABO discrepancy led to suspicion of a possible cold autoantibody based on patient histopry and diagnosis. RESt was used in an attept to adsorb the autoantobidy. The type was repeated with adsorbed plasma Anit-A=4+, Anti-B=0, A1 cells=0, B cells=0. What could be the cause of this discrepancy? - Anti-B was adsorbed out with RESt. What enzyme may be useful in distinguishing anti-Ge2? - papain The beta 1-3 linkage between galactose and N-acetylglucosamine is found in? - secretions Represents Type II chain - Beta 1-4 linkage between galactose and N acetyl glucosamine Represents decreasing concentrations of H antigen on RBCs - O>A2>B>A2B>A1>A1B The ABO genes are located on what chromosome - Chromosome 9 Which glycosyltransferase is needed before A and B antigens are formed? - L-fucosyltransferase Which reflects the A3 subgroup - mixed field reactions with Anti-A and Anti-A,B. Which fo the following reflects the A el subgroup? - Positive reactions with Anti-H What clinical condition may yield positive reactions with Anti-A, negative with Anti-B, negative with A1 and B cells. - hypogammaglobulinemia What differentiates a Bombay anti-H from an anti-H cold agglutinin? - reactivity at body temperature. This antigen is associated with linear carbohydrate chains - i antigen The following reactions are found on a 35-year old male. Anti-A=4+, Anti-B=0, Anti-A,B=4+, A1 cells=3+, B cells=4+, O cells=3+. How could these results be explained? - Anti-M in plasma Anti-IH would react best with which red blood cells? - O RBCs The acquired B phenomenon is only found in what ABO group? - A blood group A2 subgroup - Anti-A1 does not react. 1-8% of persons will produce Anti-A1 Anti-Lu3 is associated with - Recessive type Lu(a-b-) The dominant Lu(a-b-) type is associated with what gene? - In(Lu) What substance could be used to rule out anti-E in the presence of anti-Lub? - DTT Which pair of Lu alleles go together - Lu8 and Lu14 Lu6 and Lu9 Au^a and Au^b