ASCP Molecular Biology Final Certification Exam [100% PASS] /verified/

ASCP Molecular Biology Final Certification Exam [100% PASS] /verified/ Pyrimidine *Ans* One carbon ring Cytosine, Thymine, Uracil Purine *Ans* Two carbon rings Adenine, Guanine How are nucleotides joined together? *Ans* Condensation to form phosphodiester bond What is the function of mRNA? *Ans* Carries genetic info out of nucleus Transcript translated to protein What is the function of tRNA? *Ans* Carries aa to ribosome Anticodon pairs with codon on mRNA strand What is the function of rRNA? *Ans* part of ribosome structure most abundant RNA coordinated coupling of tRNA to mRNA codons Feedback inhibition *Ans* Product of pathway is noncompetitive inhibitor Binds to allosteric site to slow down rxn b/c too much product Exonucleases *Ans* Degrades nucleic acids by removing one terminal nt at a time Cleaves phosphodiester bond at end of chain 5' --> 3' and 3' --> 5' Endonucleases (Prok) *Ans* Restriction enzymes Cleaves phoshpodiester bonds w/i poly-nt chain Recognition site is palindromic sequence Types I-V ORI sites *Ans* nt sequence where replication is initiated Topoisomerase I *Ans* Induces ss breaks Remove DNA supercoils during TXN and DNA replication; for strand breakage during recombination; for chr condensation; and to disentangle intertwined DNA during mitosis topoisomerase II *Ans* cuts both strands of one DNA double helix, passes another unbroken DNA helix through it, and then reanneals the cut strands