Mock Exam I and II on Molecular Diagnostics [100% PASS] /VERIFIED/

Mock Exam I and II on Molecular Diagnostics [100% PASS] /VERIFIED/ Which of the following techniques uses probe amplification? a) SDA b) NASBA c) Invader d) bDNA *Ans* c) Invader Invader assay is a probe amplification technique, but the others are signal and target amplification techniques. Which of the following techniques uses signal amplification? a) Invader b) Hybrid Capture c) TMA d) SDA *Ans* b) Hybrid capture Hybrid capture is a signal amplification technique, but the others are probe and target amplification techniques The best wasy to remove RNase: a) bake at 100 degrees C for 4 hours b) bake at 250 degrees C for 4 hours c) 0.01% DEPC at 37 degrees C for 1 hour d) autoclave for 30 minutes *Ans* b) bake at 250 degrees C for 4 hours Baking glassware or tubes at 250 degrees C for 4 hours is the best way to remove RNase Once DNA is primed and synthesized, ______ , an enzyme that hydrolyzes RNA from a complementary DNA strand, removes the primer RNA from the short RNA-DNA hybrid, and the resulting gap is filled by another DNA polymerase, pol I. a) DNase B b) RNase H c) RNase A d) DNase H *Ans* c) RNase H RNase H is an enzyme that hydrolyzes RNA from complementary DNA strand and removes the primer RNA from the short RNA-DNA hybrid Which of the following is the correct formula for specificity? a) number of TP/number of TP + number of FN b) number of TP/ number of TP - number of FN c) number of TN / number of TN - number of FP d) number of TN / number of TN + number of FP *Ans* d) number of TN/ number of TN + number of FP Which of the following RNA polymerases will synthesize snRNA