Molecular Diagnostics Exam 3 [100% PASS] /verified/

Molecular Diagnostics Exam 3 [100% PASS] /verified/ Substitution of a nucleotide that doesn't change the amino acid sequence. *Ans* Silent Substitution changes amino acid sequence with another amino acid with similar biochemical properties. *Ans* conservative Substitution changes amino acid sequence with another amino acid with different biochemical properties. *Ans* nonconservative Terminates proteins prematurely when a nucleotide substitution produces a stop codon instead of an amino acid codon. *Ans* nonsense Insertion or deletion of more or fewer than 3 nucleotides throwing the triplet code out of frame. *Ans* frameshift ________________ is one of the most frequently used mutation screening procedure in the clinical laboratory. *Ans* single-strand conformation polymorphism SSCP Which laboratory method used to detect mutations relies on differences in melt characteristics between 2 DNA samples that have different sequences such that double-stranded DNA becomes single-stranded at different points in the gel? *Ans* denaturating gradient gel electrophoresis DGGE ________________ uses the differences in melting temperatures of short sequences of bases with one or two mismatches and those with no mismatches. *Ans* allele specific oligomer hybridization ASO In ____________, specimens with identical sequences should yield the same peak at the expected Tm; whereas, specimens containing different sequences will yield two or more peaks. *Ans* melt curve analysis The ___________________________ was designed as a method to detect SNPs in DNA. *Ans* inversion probe assay ___________________ uses solution hybridization an