COEN 6211 LECTURE 1 2021

COEN 6211 LECTURE 1 2021 Synthetic Biology Course outline Luc Varin, Ph.D (Biology) Lectures: W 14:45 – 17:30 Office Hours: Tue and Thu 13:00 – 16:00 E-mail: • Office hours: I expect students to contact me by E-mail to schedule a ZOOM session during my office hours to ask their questions related to the course material or their assignments. Course outline • Text book: This course has no text book. All the slides used in the lectures will be made available to you, on-line. Also, all of the research papers used for the latter part of the course will be available to you in PDF format. You are expected to attend every lecture, participate in it, and take your own notes. Synthetic biology Lecture 1 Course outline • Grading 1. Qualifying Exam Withdraw (0)/Pass (10) marks 2. Assignments (2) 20 marks 3. Midterm Exam 15 marks 3. Team Project 25 marks 5. Final Exam 30 marks Lecture outline Introduction to Molecular Biology • DNA structure • Gene structure • Transcription and translation • DNA replication • Gene regulation • Molecular cloning tools Flow of genetic information DNA RNA Transcription by RNA polymerase Translation by ribosomes PROTEIN Chromosome structure 7 DNA structure DNA structure DNA structure Orientation of the strands? DNA structure Nucleic acid hybridization Denaturation = dissociation of the two strands Melting = denaturation Renaturation = reassociation of the two strands Annealing = renaturation Annealing can happen between two complementary DNA strands or between DNA and RNA (hybridization) DNA structure Problem 1 5’-ACCTGCCTGACAACTG-3’ Write the sequence of the complementary strand in a 5’ to 3’ orientation DNA structure Problem 2 5’-GGATGCCT-3’ Will this oligonucleotide anneal with the DNA strand shown below? If your answer is yes, locate the position of annealing 5’-CCTACGGATCTCGGATGCCTTT-3’ DNA structure Problem 2B 5’-ATCCCAGAA-3’ Will this oligonucleotide anneal with the DNA strand shown below? If your answer is yes, locate the position of annealing 5’-CCTAGCTTCTGGGATGCCTTT-3’ DNA replication Requirements • DNA template strand • Primer with a free 3’-OH group • NTPs (ATP, TTP, CTP, GTP) • DNA polymerase G DNA synthesis CATTTTA-5’ 5’-ACTCCGCGTACGTCTAGCCTCCCGTAAAATGC-3’ DNA replication Problem 1 5’- TTCCATTCGGCGA -3’ 3’- AGC -5’ What will be the first nucleotide incorporated by DNA polymerase?