UNE Biochemistry Final Questions With 100% Correct Answers.

UNE Biochemistry Final Questions With 100% Correct Answers. The glucose-alanine cycle is a method of transferring ammonia from the skeletal muscle to the liver. In this process the non-toxic carrier of ammonia is which of the following compounds? Alanine Which of the following enzymes is correctly paired with its activator? N-acetylglutamate synthatase; arginine The urea cycle takes place in which of the following subcellular locations? the cytosol and the mitochondria Which of the following enzymes is regulated by N-acetylglutamate (NAGs)? CPS I Which of the following enzymes is the regulatory step in the urea cycle? Carbamoyl phosphate synthetase I (CPS I) Which of the following statements is consistent with nitrogen transport in the fasted state? The urea cycle will be active A comatose infant is brought to the ER. In the course of the examination, plasma ammonia was found to be elevated 20-fold over normal values. Urine orotic acid and uracil were both greater than normal. A defect of which of the following enzymes is the most likely diagnosis? Ornithine transcarbamoylase Your patient presents with a deficiency of the enzyme that catalyzes synthesis of N-acetylglutamate (NAGs). Which of the following would be a consequence of this deficiency in the patient? Increased amino acids in the blood The cellular uptake of LDL increases intracellular cholesterol levels. This has which of the following impacts on de novo cholesterol synthesis? Increases degradation of HMG-CoA reductase Dietary cholesterol is primarily transported in the body by which of the following lipoproteins? Chylomicron Secondary bile salts are generated by bacterial modification of primary bile salts in the intestinal lumen. These modifications have which of the following impacts on bile salt solubility? Decrease solubility A 47-year-old male is prescribed a statin (HMG-CoA reductase inhibitor) to manage his elevated LDL cholesterol. What is the initial mechanism of action for obtaining the desired effect in this man? Decreasing hepatic cholesterol biosynthesis A deficiency in the LDL receptor will result in an initial decrease in which of the following? Intracellular cholesterol A 45-year-old man has a mild heart attack and a history of vascular disease. He is placed on a Mediterranean diet and statin drug therapy. Which of the following will be the most likely result of this drug therapy? Low blood LDLs A 45-year-old man is found to have an elevated serum triglycerides of > 730mg/dL. He tells you he forgot to fast before his visit and he ate a full breakfast of bacon and eggs before the labs were drawn. Which of the following lipoproteins would be the largest contributors to a measurement of plasma triglycerides in this situation? Chylomicrons and VLDLs In the reaction catalyzed by cholesterol ester transfer protein (CETP), the VLDL particle receives which of the following compounds from HDL particles? Cholesterol-ester In reverse cholesterol transport HDL interacts with the ABCA1 transporter to move cholesterol from the cell to the HDL. This also requires the activity of which of the following enzyme? Lecithin cholesterol acyltransferase (LCAT) HDL particles interact with VLDL particles to exchange cholesterol ester for triacylglyerols. Which of the following enzymes is responsible for this process? Cholesterol ester transfer protein (CETP) Which of the following is a major source of extracellular cholesterol for human tissues? LDLs An individual is suspected of having a deficiency in branched chain keto acid dehydrogenase. Supplementation of which of the following vitamins or cofactors may help resolve this issue? Thiamine A 55-year old man presents to his primary care physician with bouts of dizziness and confusion. He is diagnosed with hyperhomocysteinemia due to a deficiency of cystathionine-ß synthase. In this individual which of the following amino acids could potentially become essential? Cysteine Phenylketonuria can be caused by an enzymatic deficiency or it can present due to a deficiency in which of the following required metabolic cofactors? Tetrahydrobiopterin (BH4) A 17-year old female presents to her primary care and is diagnosed with a Vitamin B12 (cobalamin) deficiency. In this individual, which of the following reactions would be compromised? Conversion of methyl malonyl-Co A to succinyl-CoA A 15-year-old female presents to her pediatrician with concerns over her inability to sleep, increased feelings of depression and generalized fatigue. A full laboratory work-up is ordered and based on these findings she is diagnosed with a melatonin deficiency. Based on this information, metabolism of which of the following amino acids is likely impaired? Tryptophan A 52-year- old male sought medical help complaining about progressive appearance of hyperchromic (hyper pigmented) papules on the edge of the second finger of both hands. He also complained about darkening of urine. Incisional biopsy of one of the spots on his hands and urine sample tested positive for or homogentisic acid. Based on these findings a diagnosis of alkaptonuria is made. This diagnosis is consistent with a deficiency in which of the following enzymes? Homogentisate oxidase Methotrexate is a potent anticancer agent that starves dividing cells of deoxyribonucleotides through direct inhibition of which of the following processes? Metabolism of folic acid A 47-year-old man complains of pain in the joints of his left big toe, which are obviously swollen and tender. The pain has been chronic but became intolerable the day after Thanksgiving when he had a large meal and several glasses of red wine. Based on this information a diagnosis of gout is made. Which of the following is involved in the pathophysiology of this patient's condition? Elevated uric acid Which of the following is the common intermediate for purine synthesis? Inosine monophosphate The ribonucleotide reductase is important for converting ribose bases to the deoxy form. This conversion is coupled with the oxidation of which of the following compounds? Thioredoxin In pyrimidine synthesis, carbamoyl phosphate synthetase II (CPSII) can be allosterically activated by which of the following compounds? 5-phosphoribosyl 1-pyrophosphate (PRPP) Which of the following enzymes is used directly in the synthesis of dTMP (thymidine)? Thymidylate synthase The regulation of PRPP synthetase is critical in the production of both purines and pyrimidines. This enzyme is inhibited under which of the following conditions? When both GDP and ADP are elevated A 4-year-old girl presented to the clinic with megaloblastic anemia and failure to thrive. Urinalysis showed significant sediment with extremely high levels of orotic acid. A deficiency in which of the following enzymes would be consistent with this presentation? Orotate phosphoribosyltransferase The transamination of all amino acids requires which of the following cofactors? Pyridoxal phosphate (PLP) Ubiquitination is a means of targeting a protein for which of the following cellular processes? Proteasomal degradation Which of the following reactions of the urea cycle takes place in the mitochondria?